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Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1993
Title:
Soilless culture.
Author:
Steiner A.A.
Year:
1968
Bibliographic source:
Proceedings, Sixth Colloquium of the International Potash Institute, Florence, Italy, pp. 324-341

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
Deviations:
yes
Remarks:
: spacing factor should be = 3; replicates should be = 4
Principles of method if other than guideline:
The toxicity of 2,4-dichloroaniline to lettuce (Lactuca sativa) was determined in soil according to OECD TG 208 and in nutrient solution according to Steiner (1968). In the first case a static and in the latter a semistatic exposure was established.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2,3-dichloro-1-nitrobenzene
- Analytical purity: = 95%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
soil test: the concentration of the test compound was analyzed at the start and at the end of the test
nutrient solution: the concentration of the test compound was analyzed just before the next replacement of the test solution

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
soil test
- all chloronitrobenzenes were either dissolved in acetone and/or mixed with a small amount of quartz sand
- afterwards the compound was mixed through the soil using the quartz sand as a carrier

nutrient solution
- all chloronitrobenzenes were dissolved in tributylalcohol

Test organisms

Species:
Lactuca sativa
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: lettuce
- Variety: Ravel R2

Study design

Test type:
early seedling growth toxicity test
Study type:
other: study in climatic chamber
Substrate type:
other: natural soil (similar to OECD TG 208) and nutrient solution (Steiner, 1968)
Limit test:
no

Test conditions

Test temperature:
21 ± 4°C
pH:
7.5
Moisture:
humidity: 40 - 80%
Details on test conditions:
GROWTH CONDITIONS
- Photoperiod: 16:8 h light:dark
- Light intensity and quality: 6,500 lx
Nominal and measured concentrations:
soil and nutrient solution
- nominal concentrations were spaced by a factor of 3.2

Results and discussion

Effect concentrationsopen allclose all
Species:
Lactuca sativa
Duration:
7 d
Dose descriptor:
EC50
Effect conc.:
20 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: growth (fresh weight of shoots)
Remarks on result:
other: 95% CL: 17-25; soil test at RIVM
Species:
Lactuca sativa
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
12 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: growth (fresh weight of shoots)
Remarks on result:
other: 95% CL: 9-17; soil test at RIVM
Species:
Lactuca sativa
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 0.32 - < 1 other: mg/l
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: growth (fresh weight of shoots)
Remarks on result:
other: nutrient solution

Applicant's summary and conclusion