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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: study well documented, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Hydrogen peroxide mutagenicity towards Salmonella typhimurium
Author:
Kensese SM, Smith LL
Year:
1989
Bibliographic source:
Teratog Carcinog Mutagen 9: 211-218

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data

Method

Species / strain
Species / strain / cell type:
other: TA97, TA98, TA100, TA102, TA1537, TA1538
Metabolic activation:
without
Test concentrations with justification for top dose:
no data

Results and discussion

Test results
Genotoxicity:
positive

Any other information on results incl. tables

Agar plate incorporation assay: positive to strain TA 102, TA 97, TA 100 and TA 1537

Liquid incubation assay: only weak mutagenicity to TA 1537 (positive effect completely abolished by catalase or superoxide dismutase)

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive

Cumene hydroperoxide is positive in the standard plate incorporation test according to Ames
Executive summary:

Cumene hydroperoxide was tested in Salmonella TA 97, TA 98, TA 100, TA 102, TA 1535 and TA 1538 in the standard plate incorporation assay according to Ames and in a liquid incubation method without metabolic activation; the substance was positive in the standard Ames test to the strains TA 102, TA 97, TA 100 and TA 1535 and showed only weak mutagenicity in the liquid incubation test in TA 1537