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EC number: 260-828-5
CAS number: 57583-34-3
The analysed radioactivity of 3H-TdR working solution was 83.72 uCi/mL
against the nominal concentration of 80 uCi/mL.
The Local Lymph Node Assay (LLNA) was conducted to evaluate the
potential of the test substance to cause contact sensitization by
measuring lymphocyte proliferative response from auricular lymph nodes
following topical application of the test item to the female CBA/Ca
Screening Study: Three daily topical application of 5, 25, 50 and 75 %
v/v in Acetone Olive oil and 100 % of the test substance were given to
one animal at each dose level. Erythema was observed at 75 and 100 %
concentration. The mice exposed to 50, 75 and 100 % concentration lost
body weights. Results from this study were used to determine the dosing
concentration in the LLNA study.
LLNA study: Six female CBA/Ca mice group received vehicle (Acetone Olive
oil [AOO]) or 30 % α-hexylcinnarnaldehyde (HCA: positive control in AOO)
or 5, 25 and 50 % of the test substance on days 1 to 3. On day 6, uptake
of 3H-methyl thymidine into the auricular lymph nodes draining the site
of test item application was measured five hours post administration.
Proper conduct of the LLNA was confirmed via a positive response using
30 % α-hexylcinnamaldehyde, contact sensitiser, which elicited
proliferation with a Stimulation Index (SI) value of 8.48, in comparison
to vehicle-treated mice. The test substance did not elicit erythema. The
test substance at the dose concentration of 50 % v/v decreased the body
weight gain. The SI values for 5, 25 and 50 % of test substance were
2.13, 7.25 and 9.05, respectively. The EC3 value was 8.40 %.
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