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EC number: 260-828-5
CAS number: 57583-34-3
The ready biodegradability of the test substance was determined using
the Manometric Respirometry Test according to Annex of EEC-Directive
92/69; corresponds to OECD Guideline 301 F and ISO Standard 9408. This
test employed biodegradation reaction mixtures containing a non-adapted
activated sludge inoculum, which was suspended in a defined mineral
medium, at a concentration of 30 mg/L (dry solids). The test substance
was added into each reaction mixture at a concentration of 50 mg/L (103
mg/L theoretical oxygen demand).
Biodegradation of the substance began at Day 4, and reached 95 ± 9.2 %
(mean ± 1 standard deviation) based on biological oxygen demand (BOD),
at the end of the 28-day test. The test substance met the 10-day window
criteria as the biodegradation reached 83.3 ± 5.9 % (mean ± 1 standard
deviation) based on BOD at Day 14 since the onset of the biodegradion at
Day 4. Since the pass level of 60 % biodegradation was achieved within
the 28-day test period and 10-day window criteria was satisfied, the
substance meets OECD criteria for “ready biodegradability” in the
Manometric Respirometry test. The results of this test met or exceeded
each of the OECD-specified criteria for validation of its ready
biodegradability tests. These include parameters such as viability of
the inoculum, control of pH and temperature, and precision in percentage
biodegradation recorded among replicate test mixtures.
Biodegradation of a reference substance (aniline) reached 90 - 100 % in
14 days, and thus verified the viability of the inoculum employed in
this test. An Inhibition (Toxicity) Control mixture, containing both
aniline (50 mg/L) and the test substance, showed no evidence for
inhibition of the microbial inoculum. An Abiotic Control mixture,
containing the test substance and a chemical sterilant (HgCl2), verified
that the observed test substance degradation was due solely to
biological activity. Thus, the test substance is considered “readily
biodegradable” according to OECD criteria. Therefore, the substance is
expected to rapidly biodegrade in a variety of environments.
Readily biodegradable in OECD 301F- Manometric respirometry test.
Biodegradation equivalent to 95 ± 9.2 % (mean ± 1 Std. Dev.) consumption
of theoretical oxygen demand (ThOD) was observed after 28 days, and 83.3
± 5.9 % (mean ± 1 Std. Dev.) biodegradation was observed within the
The test substance is considered readily biodegradable according to OECD
definition; therefore, the test substance is expected to be rapidly and
ultimately biodegradable in various environmental media.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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