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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test conducted similar to OECD TG 401; no GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report Date:
1981

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
yes
Remarks:
: no control animal group
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Methylchloroformiat (methyl chloroformate)
- Physical state: liquid
- Analytical purity: 99 %

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: HAGEMANN
- Weight at study initiation: female (160 - 200 g); male (170 - 260 g)
- Fasting period before study: 15-20 h
- Diet (e.g. ad libitum): Herilan MRH-Haltung; H . Eggersmann KG

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
olive oil
Details on oral exposure:
Concentration in vehicle: 43.00, 13.62, 9.28, 4.30, 2.94 % (w/v)
Doses:
2150, 681, 464, 215, 147 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
Statistics:
LD50-values were calculated using company software

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
313 mg/kg bw
95% CL:
229 - 427
Mortality:
147mg/kg bw: 1/10; 215mg/kg bw: 3 /10; 464mg/kg bw: 6/10; 681mg/kg bw: 10/10; 2150mg/kg bw: 10/10;
Clinical signs:
Dyspnea, apathy, staggering, tremors, piloerection, poor general state at all dose levels
Body weight:
All surviving animals gained weight
Gross pathology:
Animals that died: Heart: acute dilatation; acute passive hyperemia; stomach: hemorrhagic areas, in the forestomach in some animals ; scabbing
hemorrhagic gastritis in several animals; intestines: diffusely reddened mucosa in several animals; liver: peripheral acinar pattern sporadically
Sacrificed animals: Stomach: wall thickening at the tip of the forestomach; intraabdominal adhesion

Applicant's summary and conclusion