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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report Date:
1980

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
no
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Methylchlorformiat (methylchloroformate)
- Purity: about 99%

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Mura : SPRA (SPF 68 Han), MUS RATTUS, Brunnthal, Germany,
- Weight at study initiation: 185 +/- 15 g
- Fasting period before study:
- Housing: fully air-conditioned rooms
- Diet (ad libitum): ad libitum (except exposure); Herilan MRH of H . EGGERSMANN KG, RintelnNVeser
- Water (ad libitum): ad libitum (except exposure)

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2°C
- Humidity (%): 55 +/- 5 %
- Photoperiod (hrs dark / hrs light): 12 / 12
:

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
Exposure system: Whole-body inhalation system (Groups of 5 animals are placed in wire cages which are located in a glass-steel inhalation chamber, V= 200 I.) Generator system: Continuous infusion pump UNITA I(B . Braun); Glass evaporator with thermostat (BASF ). By means of a continuous infusion pump, constant amounts of the test substance (see Table) were supplied to an evaporator heated to 45°C . The vapors that were formed were mixed with a flow of fresh air and passed into the inhalation chamber.
By means of an exhaust air system, the pressure ratios in the inhalation system were adjusted in such a way that there was a pressure slightly below
the atmospheric pressure (negative pressure) . The inhalation mixture was offered to the animals for inhalation for 4 hours .
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
Analytical concentration: 0.006 (group 4); 0.053 (group 3); 0.120 (group 2); 0.130 mg/l (group 1)
Nominal concentration: 0.06; 0.25; 0.49; 0.37 mg/l
No. of animals per sex per dose:
10
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Weighing was before treatment, after 7 days, after 14 days. Observations were several times at the day of exposure and atleast once daily afterwards.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: daily
Statistics:
The statistical evaluation of the study was carried out based on a probit analysis of D .J . Finney (Finney, D .J. : Probit Analysis 1971, pp 1-150 .
Published by the Syndics of the Cambridge University Press, Bentley House, 200 Euston Road, London N.W. 1) . The calculations were carried out in
the BASF Computer Center.

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LC50
Effect level:
0.06 mg/L air
95% CL:
0.04 - 0.074
Exp. duration:
4 h
Sex:
male
Dose descriptor:
LC50
Effect level:
ca. 0.053 mg/L air
Sex:
female
Dose descriptor:
LC50
Effect level:
0.07 mg/L air
95% CL:
0.032 - 0.095
Mortality:
0.006 mg/l: males 0/10, females 0/10; 0.053 mg/l: males5/10, females 3/10; 0.120 mg/l: males 10/10, females 10/10;
0.130 mg/l: males 10/10, females 7/10
The fatally intoxicated animals died during the test up to 11 days afterward.
Clinical signs:
other: (Group 1-3): Dyspnea ; gasping breathing with whooping sounds in some cases; blistering in front of the noses ; reddish discharge from the eyes and noses ; incrustations around the eyes and noses occurring later ; ruffled and sticky fur with yellow discol
Body weight:
As regards the relative body weight gain, the males of Group 3 and the females of Groups 1 and 3 were considerably impaired after 7 days, but there
was an adjustment to the control groups after 14 days . The males and females of Group 4 did not reveal any substantial differences from the controlanimals .
Gross pathology:
Animals that died:
Heart: acute dilatation ; acute passive hyperemia
Lungs: infarctoid congestion, wet and sarcoid in some cases; edematized; marginal emphysema ; hydrothorax in several cases
Liver: slight and sometimes only focal peripheral hepatocellular fatty degeneration sporadically
Sacrificed animals :
Organs: no abnormalities detected

Applicant's summary and conclusion