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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20.10.2008-14.11.2008
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP compliant quideline study. A semi-static test regime was emplyed in the test involving a daily renewal of the test preparations to minimise evaporative loss.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
The test was done using a sealed system to minimise evaporative loss.
GLP compliance:
yes (incl. certificate)
Remarks:
UK, Department of Health, 15 October 2007

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): TAEE (tert-amyl ethyl ether, CAS No 919-94-8)
- Description: clear colourless liquid
- Lot/batch No.: 00931302
- Date received. 26.09.2008
- Storage conditions: room temperature in dark

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The concentration and stability of the test preparations were analysed at 0, 24, 48 and 72 hours by gas chromatography (GC-FID). Duplicate samples were taken and stored at approximately -20 C for further analysis if necessary.

Test solutions

Details on test solutions:
The test concentrations to be used in the definitive test were determined from a preliminary range-finding test. In the range-finding test fish were exposed to a series of nominal concentrations of 10, 100 and 1000 mg/l. Pre-study solubility work indicated that maximum water solubility of the test material was around 1000 mg/l and therefore this was the highest test concentration employed. All solutions were prepared in closed system to minimise evaporative loss of the the substance.

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout
- Source: Brow Well Fisheries Limited, Hebben, near Skipton, Yorkshire, UK
- Age at study initiation (mean and range, SD): juveniles
- Length at study initiation (length definition, mean, range and SD): mean lenght of 5.6 cm(+/- 0.5)
- Weight at study initiation (mean and range, SD): mean weight of 2.46 g (+/-0.71)
- Food type: commercial trout pellets
- Frequency: Feeding was discontinued approximately 24 prior to the start of test


ACCLIMATION
- Acclimation period: from 22 October to 3 November 2008
- Acclimation conditions (same as test or not):same as test
- Feeding frequency: No feeding during the test


Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
96 h

Test conditions

Hardness:
140 mg/l as CaCO3
Test temperature:
14 C
pH:
7.3-7.6
Dissolved oxygen:
7.6-10.7
Nominal and measured concentrations:
Nominal concentrations: 100, 180, 320, 560, 1000 mg/l.
Measured concentrations at time 0 hour: 83, 145, 323, 573, 1037 mg/l.
Measured concentrations at time 24 hour (old media): 86, 148, 308, 542, 983 mg/l.
Measured concentrations at time 24 hour (fresh media): 79, 164 mg/l (nominal 100, 180 mg/l)
Measured concentrations at time 48 hour (old media): 74.4, 162 mg/l (nominal 100, 180 mg/l)
Measured concentrations at time 48 hour (fresh media): 135, 85 mg/l (nominal 100, 180 mg/l)
Measured concentrations at time 72 hour (old media): 70, 133 mg/l (nominal 100, 180 mg/l)
Measured concentrations at time 72 hour (fresh media): 60, 155 mg/l (nominal 100, 180 mg/l)
Measured concentrations at time 96 hour (old media). 60, 162 mg/l (nominal 100, 180 mg/l)
Measured concentrations in control: below limit of quantification
A semistatic test regime was employed in the test involving daily renewal of the test preparations to ensure that the concentrations of the test material remained near nominal and to prevent the build up of nitrogenous waste products.
Details on test conditions:
TEST SYSTEM
- Test vessel: test vessels were completely filled and sealed
- Fill volume: 20-l glass vessel
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): daily renewal
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates):one
- No. of vessels per control (replicates):one
- Biomass loading rate: 0,86 g body weight/l

PARAMETERS MEASURED IN TEST ORGANISMS: Any mortalities and sub-lethal effects of exposure were recorded at 3, 6, 24, 48, 72 and 96 hours after the start of exposure.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:tap water, which was dechlorinated and partly softened prior to the test
- Total organic carbon: 1,041 mg/l
- Metals: Al < 13 ug/l, Fe < 13 ug/l
- Pesticides: No
- Chlorine: 0,3 mg/l
- Conductivity:542 US/cm (average)
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature, pH, and dissolved oxygen concentrations were recorded daily


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h of darkness with 20 min dawn and dusk transition periods

TEST CONCENTRATIONS

- Range finding study
- Test concentrations: 10, 100, 1000 mg/l
- Results used to determine the conditions for the definitive study: no mortality nor sub-lethal effects of exposure were during the range finding test

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
240 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 180-320 mg/L
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: None detected
- No observations on body lenght and body weight were included in the test
- Other biological observations: No other observations made
- Mortality of control: None
- Other adverse effects control: No other adverse effect present
- Abnormal responses: None present
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None present
- Effect concentrations exceeding solubility of substance in test medium: No

Any other information on results incl. tables

Although some of the samples showed measured concentrations outside the 80 % to 120 % acceptance limits, the overall mean measured concentration was calculated to be 86% of nominal. Therefore it was considered justifiable to base the results on nominal test concentrations only.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Results from an acute toxicity of the TAEE on rainbow trout gave a 96-h LC50 value of 240 mg/l. The No Observed Effect Concentration was 100 mg/l.
Executive summary:

A short term toxicity test of TAEE to rainbow trout was performed in GLP-compliant laboratory according to OECD Guidelines for Testing Chemicals (1992) No 203, "Fish, acute toxicity test". Test organisms (juvenile rainbow trout;Oncorhynchus mykiss) were exposed to the test material at six nominal concentration (100, 180, 320, 560 and 1000 mg/l) in a a semi-static 96 hour test. Any mortalities and sub-lethal effects on exposure were recorded at 3, 6, 24, 48, 72, and 96 hours after the start of exposure. The test vessels were sealed to minimise evaporative losses from the system. The overall mean measured concentration was calculated to be 86% of nominal and it was considered justifiable to base the results on nominal test concentrations only. The test material gave a 96 -hour LC50 value of 240 mg/l with confidence limits of 180 -320 mg/l. The No Observed Effect Concentration was 100 mg/l. Based on these data, TAEE is not acutely toxic to freshwater fish (LC50>100 mg/L).