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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
September 16-19, 2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study is made in GLP laboratory according standardised methods.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
The test substance was received at Brixham Environmental Laboratory on 1 May 2009. Purity of sample was 99.4%, measured by GC.The sample was stored in the dark at ambient temperature, in the container in which it was received until required for testing, when an appropriate sub-sample was provided for the test operator.

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
This study was run with a dilution water control (DWC) and nominal exposure concentrations of 6.8, 22, 68, 220 and 680 mg/L. The nominal TAEE concentrations of 68, 220 and 680 mg/L were prepared by direct addition of 92, 295 and 900 μl of the test substance to 1000 ml of dilution water respectively. The test substance was added to a gently stirring vortex and then stirred for 30 minutes. The resultant solutions were clear and colourless with a tiny globule of material on the stirrer bar of 220 and 680 mg/L.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: laboratory cultures of Brixham Environmental Laboratory
- temperature 20+/- 2 C
- light: 16 hours light, 8 hours dark, 20 min transition period
- Age at study initiation: < 24 hours
- The parent animals were 22+-1 days old, twice weekly renewal of water medium since birth
- Reproduction by diploid parthenogenesis.
- Culture showed no evidence of disease
Feeding during test:
- Food type: Chlorella vulgaris, strain CCAP 211/12 and Pseudokirchneriella subcabitata, strain ATCC 22662
- Amount: ad libitum
- Frequency: daily

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
24 h and 48 h measured

Test conditions

Hardness:
Total hardness as CaCO3 251.3 mg/l
Test temperature:
20.0+/- 1 C
0 h, 20.0 C
24 h, 20.6 C
48 h, 20.0 C
pH:
7.79 - 7.95
Dissolved oxygen:
8.7 - 9.4 mg/l
Salinity:
Conductivity 639 µS/cm
Nominal and measured concentrations:
The mean measured concentration of TAEE (tert-amyl ethyl ether) ranged from 67-106 % of the nominal concentrations. Consequently, the mean measured concentrations were used for the calculation and reporting of results.
Details on test conditions:
TEST SYSTEM
- Test vessel: glass bottels
- Type: closed, Teflon faced disc/crimp closures
- Material, size, headspace, fill volume: glass, 50 ml
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4
Dilution water parameters
- Source/preparation of dilution water: Elendt's M4 Daphnia magna medium
*pH 8.02
*Conductivity @ 25ºC 639 μS cm-1
*Total hardness as CaCO3 251.3 mg l-1
*Alkalinity as CaCO3 32.8 mg l-1
Total suspended solids <3.0 mg l-1
Dissolved organic carbon 0.70 mg l-1
Chemical oxygen demand <12.0 mg l-1
Ammoniacal nitrogen as N <0.002 mg l-1
Highest organophosphorous pesticides <0.01 μg l-1
Highest organochlorine pesticides <0.03 μg l-1
Highest PCB <0.001 μg l-1
Aluminium <10.0 μg l-1
Arsenic <1.00 μg l-1
Boron 476 μg l-1
Cadmium <0.1 μg l-1
Chromium <0.5 μg l-1
Cobalt 2.0 μg l-1
Copper 6.8 μg l-1
Iron 143 μg l-1
Lead <2.0 μg l-1
Manganese <10.0 μg l-1
Mercury <0.01 μg l-1
Nickel <1.0 μg l-1
Silver <1.0 μg l-1
Zinc 7.3 μg l-1

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours dark, 20 min transition
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 143.2 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Remarks:
TAEE
Basis for effect:
mobility
Remarks on result:
other: 95% conf interval 108.6-194.2 mg/l
Details on results:
There was no immobility observed in the dilution water control. Other symptoms of toxicity were observed at 24 hours in the nominal 220 mg l-1 where 30% of the D.magna were alive, very slow moving and exhibiting twitching behaviour. Of these, 67% of them had debris stuck to their body appendages.

Any other information on results incl. tables

Time (hours) Nominal conc of TAEE (mgl-1) Mean measured conc of TAEE (mgl-1) Number immobilised per replicate Total number tested Total number immobilised Persentage immobilised
A B C D
24 DWC DWC 0 0 0 0 20 0 0
6.8 8.7 0 0 0 0 20 0 0
22 28 0 0 0 0 20 0 0
68 84 0 0 0 0 20 0 0
220 340 3 3 4 4 20 14 70
680 670 5 5 5 5 20 20 100
48 DWC DWC 0 0 0 0 20 0 0
6.8 8.7 0 0 0 0 20 0 0
22 28 0 0 0 0 20 0 0
68 84 0 0 0 0 20 0 0
220 340 5 4 5 5 20 19 95
680 670 5 5 5 5 20 20 100

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Results from an acute toxicity of the TAEE on Daphnia magna (immobilisation test) gave a 48-h EC50 value of 143.2 mg/L. The No Observed Effect Concentration was 84 mg/L.
Executive summary:

The acute toxicity of TAEE (tert-amyl ethyl ether) to Daphnia magna was tested in 2009 by Brixham Environmental Laboratory, UK. The test was static 48 hours test with nominal concentration 10,32,100,320 and 1000 mg/L, and dilution water control. Results based on mean measured concentrations of TAEE are EC50 (24 h) 186.8 mg/L and EC50 (48 h) 143.2 mg/L. 48 hours no observedd effect concentration (NOEC) based on immobility is 84 mg/L.