Registration Dossier

Administrative data

Endpoint:
biodegradation in soil
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP compliant, no guideline study, adequate for assessment. However, no analytical purity of the tested TAME sample was reported.

Data source

Reference
Reference Type:
publication
Title:
Investigation of the intrinsic biodegradation of alkyl and cyclic ethers
Author:
Zenker MJ
Year:
1999
Bibliographic source:
5th International in situ On-site Bio-remediation Symposium, volume 1, pp 165 - 170, editors Alleman B and Leeson, A, publ. Battelle Press, Colombus, Ohio

Materials and methods

Principles of method if other than guideline:
Biodegradation was evaluated in natural soils under aerobic conditions.
GLP compliance:
no
Test type:
laboratory

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity not specified in the publication
Radiolabelling:
no

Study design

Oxygen conditions:
aerobic
Soil classification:
other: natural soil
Details on soil characteristics:
No details given
Duration of test (contact time)
Soil No.:
#1
Duration:
> 300 d
Initial test substance concentration
Soil No.:
#1
Initial conc.:
3 000 other: µg/L
Based on:
test mat.
Details on experimental conditions:
All microcosms were constructed of amber 255 mL screw-top bottles sealed with Teflon Miniert valves (Suelco Inc.). Each bottle contained 150 grams of wet soil, 140 mL of sterile groundwater, and the chemical. All utensils, jars and tools used in microcosm assembly were autoclaved prior to use. Once constructed, the microcosms were analyzed for the contaminant and dissolved organic carbon (DOC) every thirty day.
All treatments were conducted aerobically and incubated in the dark at 16 °C. Each treatment was constructed in triplicate with mathcin abiotic controls. The soil used for the abiotic controls was autoclaved for one hour on each of three consecutive days. Further, 250 mg/L of mercuric chloride was added to ensure no biological activity.

Results and discussion

% Degradation
Soil No.:
#1
% Degr.:
ca. 0
Parameter:
test mat. analysis
Sampling time:
1 yr
Transformation products:
no
Details on transformation products:
No data
Evaporation of parent compound:
no
Volatile metabolites:
no
Residues:
no
Details on results:
No biodegradation of TAME after one year was observed.
Results with reference substance:
No data

Any other information on results incl. tables

No degradation was observed in soil after 1 year of incubation

Applicant's summary and conclusion