(Z)-3,7-dimethylocta-2,6-dienal

Toxicity to reproduction: other studies

Administrative data

Endpoint:

toxicity to reproduction: other studies

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data meet generally accepted scientific principles with the following restriction: only basic data available from short information on the study, no information on test protocol available

Data source

Reference

Reference Type:

other: short information

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Principles of method if other than guideline:

Uterotrophic assay: uterus weights as indicator of estrogen-like activity

GLP compliance:

not specified

Type of method:

in vivo

Test material

Test material information

Constituent 1

Reference substance name:

Citral

EC Number:

226-394-6

EC Name:

Citral

Cas Number:

5392-40-5

IUPAC Name:

3,7-dimethylocta-2,6-dienal

Constituent 2

Reference substance name:

(Z)-3,7-dimethylocta-2,6-dienal

EC Number:

203-379-2

EC Name:

(Z)-3,7-dimethylocta-2,6-dienal

Cas Number:

106-26-3

Molecular formula:

C10H16O

IUPAC Name:

(2Z)-3,7-dimethylocta-2,6-dienal

Details on test material:

- Name of test material (as cited in study report): Citral
- Lot/batch No.: 80-0087

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Administration / exposure

Route of administration:

oral: unspecified

Duration of treatment / exposure:

3 d

Frequency of treatment:

daily

Duration of test:

3d

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 300, 1000 mg/kg bw/d
Basis:

No. of animals per sex per dose:

10

Control animals:

yes

Details on study design:

Positive control: DES-DP (5 µg/kg bw/day)

Statistics:

Dunnett's test

Results and discussion

Observed effects

Clinical findings: nothing abnormal detected
Uterus weight: The significant reduction of uterus weights in case of 1000 mg citral/kg bw treatment was judged as not biologically relevant. The sensitivity of the rats was demonstrated by the induced estrogen-like effect with the positive control substance DES-DP.

Any other information on results incl. tables

Table 1: Test results from uterotrophic assay

Group	Negative control	Positive control (DES-DP) µg/kg	Citral mg/kg bw/day
Dose	0	5	300	1000
Animal numbers	10	10	10	10
Clinical findings	NAD	NAD	NAD	NAD
Body weight gain (g)	14.5	14.1	14.3	13.3
Uterus weight
absolute (g)	0.039	0.120**	0.036	0.033*
relative (%)	0.069	0.219	0.065	0.061

* p≤0.05; ** p≤0.01 (Dunnett-test)

NAD: nothing abnormal detected