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In a reliable (RL 1) activated sludge respiration inhibition test according to OECD 209 no statistically significant inhibition of respiration rate was observed for the submission substance. No EC10 or EC50 could be determined and the NOEC (3h, respiration inhibition) is equal or larger than 1000 mg/L nominal concentration.

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A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 and compliant to GLP (RL 1) was carried out as a limit test at the nominal limit concentration of 1000 mg/L submission substance.The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours. The inhibition of respiration for the test item replicates ranged from -7 % (stimulation) to 4 % and were not statistically significant. Thus, the determined NOEC (3h, respiration inhibition) for the submission substance is equal or larger than 1000 mg/L nominal concentration. The activity of the test system and the test conditions was demonstrated with the reference test item copper (II) sulphate pentahydrate.