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Long-term toxicity to aquatic invertebrates

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long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Study period:
January 23rd - June 12th, 2013
1 (reliable without restriction)

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-oxybis[5,5-dimethyl-1,3,2-dioxaphosphorinane] 2,2'-disulphide
EC Number:
EC Name:
2,2'-oxybis[5,5-dimethyl-1,3,2-dioxaphosphorinane] 2,2'-disulphide
Cas Number:
Molecular formula:
2,2'-oxybis[5,5-dimethyl-1,3,2-dioxaphosphorinane] 2,2'-disulphide
Details on test material:
- Name of test material (as cited in study report): Exolit 5060 PK
-Molecular formula: C10H20O5P2S2
- Physical state: solid matter
- Composition of test material, percentage of components:
2,2'-oxybis[5,5-dimethyl-1,3,2 dioxaphosphorinana]2,2'disulphide: 100.1 +/- 1.1%
Pyridine: <40 ppm
Water: 0.02%
Oily component: 0.1%
- Lot/batch No.: CHA0104008
- Expiration date of the lot/batch: January 8th, 2016
- Stability under test conditions: stable
- Storage condition of test material: store in sealed original container at a coll, dry and well ventilated place
- Other:

Sampling and analysis

Analytical monitoring:
Details on sampling:
- Concentrations: 6.25, 12.5, 25, 50, 100 mg/L
- Sampling method: sampling took place at the start and at the end of the test and two times in between (once a week) with change of medium from freshly prepared beakers and at the time of renewal
- Sample storage conditions before analysis: the samples were stored in the deep freezer at < -18°C until dispatch

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Due to the sparingly soluble properties of the test item, the study was performed with the water accommodated fraction (WAF) prepared according to the OECD Series on Testing and Assessment Number 23: "Guidance Document on Aquatic Toxicity of Difficult Substances and Mixtures": The WAF was prepared with M4-Medium. The WAF was stirred for 48h at 20 - 21°C in diffuse light. For stirring an overhead high grade stainless steel radial impeller was used (about 450 rpm). The impeller was placed about 7 cm above the bottom of the beaker. After stirring was stopped, the suspension was allowed to float and sediment for a period of 1 hour. After stopping the stirring the WAF was slightly turbid, but after sedimentation and flotation period it was clear and no particles could be observed anymore.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Daphnia magna STRAUS
- Source: German Federal Environmental Agency, Department V 3.2, Berlin
- Feeding during test
- Food type: Desnidesnzs szbspicatus
- Amount: from day 0 to day 6: 0.1 mg C/Daphnia/day; from day 7 to day 21: 0.2 mg C/Daphnia/day
- Frequency: weekdaily

Study design

Test type:
Limit test:
Total exposure duration:
21 d

Test conditions

not determined
Test temperature:
20.5 - 21.4 °C
7.4 - 8.2
Dissolved oxygen:
7.0 - 8.4
not determined
Nominal and measured concentrations:
Nominal concentrations:
6.25; 12.5; 25; 50 and 100 mg/L
Measured concentrations:
Almost all DOC-values are near or under the lower detection limit of the total carbon analyser.
Measured DOC-values for nominal concentration 100 mg/L: 25.3 - 35.0 µg/L
Measured DOC-values for nominal concentration 50 mg/L: 22.0 - 30.7 µg/L
Measured DOC-values for nominal concentration 25 mg/L: 19.9 - 28.2 µg/L
Measured DOC-values for nominal concentration 12.5 mg/L: 13.8 - 24.4 µg/L
Measured DOC-values for nominal concentration 6.25 mg/L: 13.0 - 18.7 µg/L
Details on test conditions:
- Test vessel: Beakers à 100 ml, Schott, Mainz, Germany
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Duran glass, 100 ml, 25 ml headspace, 75 ml fill volume
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): three times a week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

- Source/preparation of dilution water: Elendt-M4-medium according to OECD 202
- Culture medium different from test medium: no

- Adjustment of pH: no
- Photoperiod: 16h
- Light intensity: 6.73 µmol/m2

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
weekdaily the offspring produced by each parent animal were removed and counted from the appearance of the first brood. If dead parent daphnia were observed these were counted and removed.
At the end of the test the measurement of the total length of each parent animal were determined


Results and discussion

Effect concentrations
21 d
Dose descriptor:
Effect conc.:
>= 100
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Details on results:
- Mortality of parent animals: yes
control: one parent daphnia died
6.25 mg/L: two daphnia died
25 mg/L: one daphnia died
- No. of offspring produced per day per female:
control: 6.1
6.25 mg/L: 5.1
12.5 mg/L: 6.1
25 mg/L:5.8
50 mg/L: 6.1
100 mg/L: 5.4

- Body length and weight of parent animals:
control: 6.929 mm
6.25 mg/L: 6.836 mm
12.5 mg/L: 6.910 mm
25 mg/L: 7.007 mm
50 mg/L: 7.002 mm
100 mg/L: 6.938 mm

- Time to first brood release or time to hatch: in all concentrations and in the control the age of first reproduction of the parental animals was 10.5 days
- Effect concentrations exceeding solubility of substance in test medium: The data provided from the sponsor indicated a water solubility of the test item of about 1.4 mg/L. In the WAFS after 48 hours stirring the measured DOC was in the range of the limit of quantifiction . The test item is poorly soluble in the test medium under test conditions.

Any other information on results incl. tables

Mean Cumulative Offspring per Survivor at 21.0 d:

Treatm.[mg/L] Mean Std. Dev. n %Reduction
Control 127,0 19,90 9 0
6,3 111,0 24,80 8 12,6
12,5 129,0 20,00 10 -1,6
25,0 121,0 10,80 9 4,5
50,0 127,0 16,20 10 -0,5
100,0 113,0 22,70 10 10,6

Due to the low water solubility the test item was tested as “water accommodated fractions” (WAFs) at 5 nominal concentrations between 6.25 mg/L and 100 mg/L.

Concerning the mean cumulative offspring per survivor the results are:

EL50 (Effective Loading rate) is > 100 mg/L

LOELR (Lowest Effective Loading Rate)> 100 mg/L

NOELR (No Observed Effect Loading Rate) ≥ 100 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
Executive summary:

Due to the low water solubility the test item was tested as “water accommodated fractions” (WAFs) at 5 nominal concentrations between 6.25 mg/L and 100 mg/L.

Concerning the mean cumulative offspring per survivor the results are:

EL50 (Effective Loading rate) is > 100 mg/L

LOELR (Lowest Effective Loading Rate)> 100 mg/L

NOELR (No Observed Effect Loading Rate) ≥ 100 mg/L