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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Test was conducted according to OECD test guideline in accordance with GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Salicylaldehyde
EC Number:
201-961-0
EC Name:
Salicylaldehyde
Cas Number:
90-02-8
Molecular formula:
C7H6O2
IUPAC Name:
salicylaldehyde
Details on test material:
Name of test material (as cited in study report): 2-hydroxybenzaldehyde - Analytical purity:> 97.0 % - Impurities (identity and concentrations): water, < 0.2 % - Lot/batch No.: FGC01 - Stability under test conditions: IR chromatogrammes were measured before and after the experiment.- Storage condition of test material: Cooled place 

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
From 10 different places including sewage treatment plants, rivers, lake and inner sea in Japan, sludge and environmental water and soil were collected in September 1996. Then, these samples were mixed and cultivated with glucose, peptone, potassium dihydrogenphosphate. Concentration of the sludge was 30 mg/L as suspended solid matters.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Details on study design:
6 bottles were prepared, which are as follows. [Water + Test substance][Test medium + Test substance + Sludge] -1 [Test medium + Test substance + Sludge] -2 [Test medium + Test substance + Sludge] -3 [Test medium + Sludge]Volume of test medium or water: 300 mL Volume of the test substance added was 30.3 mg which is injected with 29. 5 uL by micro-syringe. 30.3 mg = 29.5 uL x 1.022 g/cm3 (density)BOD was continuously measured by BOD meter over the cultivation period. After the cultivation period, TOC was measured. Direct analysis by HPLC was also conducted after the cultivation period.
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
other: BOD
Value:
2
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Degradability of 2-hydroxybenzaldehyde by BOD was 2 % according to OECD Test guideline 301C. However, 2-hydroxybenzaldehyde was not detected in the test system and whole amount of 2-hydroxybenzaldehyde was converted to the transformation product, 2-hydroxybenzoic acid (salicylic acid) after the cultivation period.

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