Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD test guideline study in compliance of GLP and with analytical measurements of the chemical.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Salicylaldehyde
EC Number:
201-961-0
EC Name:
Salicylaldehyde
Cas Number:
90-02-8
Molecular formula:
C7H6O2
IUPAC Name:
salicylaldehyde
Details on test material:
salicylaldehyde (CAS: 90-02-8, AGC SEIMI Chemical Co., Ltd., Lot. No.; 041208, Purity =99.8 %, Impurity = 0.2% (unclear))

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.6 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
mobility

Any other information on results incl. tables

- Measured Concentrations: The test concentrations were measured at 0, 24 (before and after water replacement), and 48-hour using HPLC.Table1. Concentration of the Test Substance in Test Water.

Nominal Conc.(mg/L)

Concentration (mg/L)(Percent of Nominal, %)

Time-weighted Mean (mg/L)

0 hr

24 hr(old)

24 hr(new)

48 hr

Control

---

---

---

---

---

2.02

1.872 (92.7)

0.595 (29.5)

1.88 (93.1)

0.061 (3.0)

0.82

2.63

2.428 (92.3)

0.774 (29.4)

2.456 (93.4)

0.123 (4.7)

1.11

3.41

3.116 (91.4)

1.141 (33.5)

3.231 (94.8)

0.170 (5.0)

1.50

4.44

4.164 (93.8)

1.491 (33.6)

4.245 (95.6)

0.267 (6.0)

2.02

5.77

5.331 (92.4)

2.222 (38.5)

5.508 (95.5)

0.529 (9.2)

2.84

7.50

6.998 (93.3)

3.212 (42.8)

7.119 (94.9)

0.727 (9.7)

3.83

- Water Chemistry: Water chemistry and temperature were measured for control and each concentration at 0, 24 (before and after water replacement), and 48-hour.pH: 7.3 – 7.6.DO: 6.7 – 7.4 mg/L.Water Temperature: 19.4 – 19.6 C.-Effect Data:EC50 (48hr) 2.60 mg/L (mc).95% Confidence Interval: 2.38 – 2.84 mg/L.EC0: 1.50 mg/L.EC100: 3.83 mg/L.mc: based on measured concentrations.-Mortality or Immobility: None of test organisms were immobilized the behaviours at control, 0.82, and 1.50 mg/L.Table2. The numbers of Immobile Daphnia magna (Percent Immobility).

Nominal Conc.(mg/L)

Measured Conc. (mg/L)

Cumulative Number of Immobilized Daphnia (Percent Immobility, %)

24 hr

48 hr

Control

---

0 (0)

0 (0)

2.02

0.82

0 (0)

0 (0)

2.63

1.11

0 (0)

0 (0)

3.41

1.50

0 (0)

0 (0)

4.44

2.02

0 (0)

1 (5)

5.77

2.84

6 (30)

15 (75)

7.50

3.83

14 (70)

19 (95)

Applicant's summary and conclusion

Categories Display