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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD test guideline study in compliance of GLP and with analytical measurements of the chemical.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Automatically generated during migration to IUCLID 6, no data available
IUPAC Name:
Automatically generated during migration to IUCLID 6, no data available
Details on test material:
salicylaldehyde (CAS: 90-02-8, AGC SEIMI Chemical Co., Ltd., Lot. No.; 041208, Purity =99.8 %, Impurity = 0.2% (unclear))

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
4.8 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
1.6 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
other: area under growth curve
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.55 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.55 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
other: area under growth curve

Any other information on results incl. tables

- Measured Concentrations: Test concentrations were measured at the start and the end of test using by HPLC.

Table1. Concentration of the Test Substance in Test Water.

Nominal Conc. (mg/L)

Measured Concentration (mg/L)(Percent of Nominal, %)

Geometric mean(mg/L)

0 hour (new)

72 hours (old)

Control

-

-

--

1.0

0.81 (81)

0.37 (37)

0.55

2.2

1.74 (79)

0.83 (38)

1.20

4.8

3.56 (74)

1.97 (41)

2.65

10.6

8.47 (80)

5.36 (51)

6.74

23.4

19.66 (84)

13.49 (58)

16.29

- Water Chemistry: pH was measured for controls and each concentration. At the start and the end of test, the pH was 7.8 - 8.0 and 7.9 - 9.3, respectively. Temperature in algal culture cabinet was maintained at 23 +/- 2 C during test period.

pH: 7.8 - 9.3,Temperature: 23.0 – 23.1 C.

Table2. pH Values.

Nominal Conc. (mg/L)

pH

0hour

72hour

Control

8

9.3

1.0

8

9

2.2

8

8.4

4.8

8

8

10.6

7.9

7.9

23.4

7.8

7.9

-Effect Data: Growth Rate Method

EC50 (0 - 72 hr): 4.76 mg/L (mc), 95% Confidence Interval: 4.27 – 5.34 mg/L

NOEC (0 - 72 hr): 0.55 mg/L (mc)

mc: based on measured concentration

-Effect Data: Area Method (area under growth curve)

EC50 (0 - 72 hr): 1.57 mg/L (mc), 95% Confidence Interval: 1.43 – 1.72 mg/L

NOEC (0 - 72 hr): 0.55 mg/L (mc)

- Growth Inhibition (%) of Pseudokirchneriella subcapitata.

Table3. Growth Rate, Inhibition and Cell density of Pseudokirchneriella subcapitata.

Measured Conc. (mg/L)

Growth Rate, Inhibition and Cell density

Rate (Average)

Inhibition (%)

Cell density (cells/ml)

u(0-72hr)

Iu (0-72hr)

(72hr)

Control

1.33

-

546,000

0.55

1.33

0.23

540,333

1.20

1.14

14.8

302,333

2.65

0.88

34.2

139,000

6.74

0.60

55.1

61,267

16.29

0.15

88.6

16,133

- Growth Curves: Exponential growth phase during 72 hours.

Measurement of algal biomass was not conducted in this test. Present values were obtained and estimated from growth rate based on number of algal cells.

Applicant's summary and conclusion