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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Metabolic dinitrosation of diphenylnitrosamine: a possible bioactivation pathway
Author:
Appel KE, Görsdorf S, Scheper T, Ruf HH, Rühl CS, Hildebrandt AG.
Year:
1987
Bibliographic source:
J.Cancer Res.Clin.Oncol. 113: 131:136

Materials and methods

Objective of study:
metabolism
Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The metabolism of nitrosodiphenylamine was investigated in phenobarbital-induced mouse liver microsomes and some of the metabolites were also tested.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Nitrosodiphenylamine
EC Number:
201-663-0
EC Name:
Nitrosodiphenylamine
Cas Number:
86-30-6
Molecular formula:
C12H10N2O
IUPAC Name:
N-nitroso-N-phenylaniline
Radiolabelling:
no

Test animals

Species:
other: mice liver cells

Results and discussion

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
Besides the parent compound, three metabolites were found, one of which was diphenylamine. The two other metabolites were identified as 4-hydroxydiphenylamine and its oxidized product, the corresponding quinoneimine.

Applicant's summary and conclusion

Executive summary:

The metabolism of nitrosodiphenylamine was investigated in phenobarbital-induced mouse liver microsomes and some of the metabolites were also tested.

One metabolite was identified as diphenylamine whereas the others were identified as a ring-hydroxylated derivative of diphenylamine and its corresponding quinoneimine.