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EC number: 201-663-0 | CAS number: 86-30-6
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- Two concentrations of n-nitrosodiphenylamine (5 and 10 mg/L) were added to a culture medium containing domestic water inoculum. Degradability was assessed by both DOC and TOC methods after 7 days. In addition, it was followed by 3 weekly subcultures. In brief, a sample of the activated inoculum of the preceding incubation flasks was added to new flasks containing fresh incubation medium and both concentration of test-item (5 and 10 mg/L). After 7 days, biodegradability was assessed and so on.
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- No data
- Duration of test (contact time):
- 7 d
- Initial conc.:
- 5 mg/L
- Based on:
- DOC
- Initial conc.:
- 10 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- TOC removal
- Details on study design:
- TEST CONDITIONS
- Solubilising agent (type and concentration if used): ethanol, concentration not mentioned
- Test temperature: 25°C
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Erlenmeyer flask
- Number of culture flasks/concentration: 3 per concentration
- Method used to create aerobic conditions: static, cotton stoppered
- Measuring equipment: GC
- Test performed in closed vessels due to significant volatility of test substance: no
SAMPLING
- Sampling frequency: once a week
- Sampling method: not mentioned
- Sample storage before analysis: not mentioned
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
STATISTICAL METHODS: not mentioned - Reference substance:
- other: phenol
- Parameter:
- other: DOC or TOC removal
- Value:
- 87
- Sampling time:
- 7 d
- Remarks on result:
- other: 1st flask (1st week) - 5 mg/L
- Parameter:
- other: DOC or TOC removal
- Value:
- 100
- Sampling time:
- 7 d
- Remarks on result:
- other: 2nd flask (2nd week) - 5 mg/L
- Parameter:
- other: DOC or TOC removal
- Value:
- 92
- Sampling time:
- 7 d
- Remarks on result:
- other: 3rd flask (3rd week) - 5 mg/L
- Parameter:
- other: DOC or TOC removal
- Value:
- 100
- Sampling time:
- 7 d
- Remarks on result:
- other: 4th flask (4th week) - 5 mg/L
- Parameter:
- other: DOC or TOC removal
- Value:
- 47
- Sampling time:
- 7 d
- Remarks on result:
- other: 1st flask (1st week) - 10 mg/L
- Details on results:
- %degr - 1st flask - 10 mg/L: 43%
%degr - 2nd flask - 10 mg/L: 63%
%degr - 3rd flask - 10 mg/L: 95%
%degr - 4th flask - 10 mg/L: 98% - Results with reference substance:
- Phenol was biodegraded at 100% or almost 100% (>96%) for both concentrations in all trials.
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Based on the results, the substance is considered inherently biodegradable.
- Executive summary:
Biodegradability of n-nitrosodiphenylamine was recorded according to a non standard method. Two concentrations of n-nitrosodiphenylamine (5 and 10 mg/L) were added to a culture medium containing domestic water inoculum. Degradability was assessed by both DOC and TOC methods after 7 days. In addition, it was followed by 3 weekly subcultures. In brief, a sample of the activated inoculum of the preceding incubation flasks was added to new flasks containing fresh incubation medium and both concentration of test-item (5 and 10 mg/L). After 7 days, biodegradability was assessed and so on.
After 7 days, the smallest biodegradation was 47% and the highest was 100%. The test-item is considered inherently biodegradable and not readily biodegradable because a study on degradation simulation in soil showed that nitrosodiphenylamine is not rapidly biodegradable (half-life of 45 days at 12°C).
Reference
Description of key information
Although some degradation can be observed, nitrosodiphenylamine is not readily biodegradable. This substance is however inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
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