Nitrosodiphenylamine

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Radiolabelling:

no

Details on study design: HPLC method:

The test system consisted of a mass spectrometer fitted with a time of fly mass analyzer and a UV spectrometer placed in series.

UPLC System: Waters UPLC System including binary solvent manager, sample manager, column heater and PDA detector
LCT Premier Mass Spectrometer
Column: Agilent technologies ZORBAX CN 5µm, dim: 2.1 x 150 mm
Column temperature: 30ºC
Mobile phase: Methanol : Water (55.0:45.0 v/v)
pH of mobile phase: 6.5 at 25°C, 6.6 at 30°C
Flow-rate: 0.8 mL/min
Injection volume: 5 µL
UV detector wavelength: 210 nm

Key result

Sample No.:

#1

Type:

log Koc

Value:

3.02 L/kg

pH:

6.5

Temp.:

25 °C

Details on results (HPLC method):

- Retention times of reference substances used for calibration: see table 1
- Details of fitted regression line (log k' vs. log Koc): linear regression, r² = 0.97
- Average retention data for test substance: see table 2

Table 1: Reference items results

Name	Mean retention time (min)	Log k'	Log Koc
Formamide	0.53	NA	NA
Acetanilide	0.7	-0.49382695	1.25
Methyl benzoate	0.86	-0.20576193	1.80
Triadimenol	1.07	0.00811789	2.40
Naphtalene	1.29	0.15653772	2.75
phenanthrene	2.27	0.51627338	4.09
DDT	4.44	0.86790089	5.63

  

Table 2: Test item results

Measure	Retention time (min)	Log k'	Log Koc	Average Log Koc
1	1.28	0.15078539	3.02	3.02
2	1.28	0.15078539	3.02
3	1.28	0.15078539	3.02

 

Validity criteria fulfilled:

yes

Conclusions:

Log Koc of nitrosodiphenylamine is 3.02 L/kg

Executive summary:

An OECD 117 GLP compliant study was carried out to determine the koc of nitrosodiphenylamine. Retention time of nitrosodiphenylamine was plotted against a set of reference compounds. The linear regression of the reference compounds fitted correctly (r²=0.97). Log Koc of nitrosodiphenylamine was determined in triplicate. The coefficient of variation of the measure was 0. Log Koc = 3.02 L/kg

Description of key information

According to an OECD 121 GLP compliant study, log Koc of nitrosodiphenylamine is 3.02

Key value for chemical safety assessment

Koc at 20 °C:

1 047

Additional information

[LogKoc: 3.02]