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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
HPLC estimation method
Radiolabelling:
no
Details on study design: HPLC method:
The test system consisted of a mass spectrometer fitted with a time of fly mass analyzer and a UV spectrometer placed in series.

UPLC System: Waters UPLC System including binary solvent manager, sample manager, column heater and PDA detector
LCT Premier Mass Spectrometer
Column: Agilent technologies ZORBAX CN 5µm, dim: 2.1 x 150 mm
Column temperature: 30ºC
Mobile phase: Methanol : Water (55.0:45.0 v/v)
pH of mobile phase: 6.5 at 25°C, 6.6 at 30°C
Flow-rate: 0.8 mL/min
Injection volume: 5 µL
UV detector wavelength: 210 nm
Key result
Sample No.:
#1
Type:
log Koc
Value:
3.02 L/kg
pH:
6.5
Temp.:
25 °C
Details on results (HPLC method):
- Retention times of reference substances used for calibration: see table 1
- Details of fitted regression line (log k' vs. log Koc): linear regression, r² = 0.97
- Average retention data for test substance: see table 2

Table 1: Reference items results

Name

Mean retention time (min)

Log k'

Log Koc

Formamide

0.53

NA

NA

Acetanilide

0.7

-0.49382695

1.25

Methyl benzoate

0.86

-0.20576193

1.80

Triadimenol

1.07

0.00811789

2.40

Naphtalene

1.29

0.15653772

2.75

phenanthrene

2.27

0.51627338

4.09

DDT

4.44

0.86790089

5.63

  

Table 2: Test item results

Measure

Retention time (min)

Log k'

Log Koc

Average Log Koc

1

1.28

0.15078539

3.02

3.02

2

1.28

0.15078539

3.02

3

1.28

0.15078539

3.02


 

Validity criteria fulfilled:
yes
Conclusions:
Log Koc of nitrosodiphenylamine is 3.02 L/kg
Executive summary:

An OECD 117 GLP compliant study was carried out to determine the koc of nitrosodiphenylamine. Retention time of nitrosodiphenylamine was plotted against a set of reference compounds. The linear regression of the reference compounds fitted correctly (r²=0.97). Log Koc of nitrosodiphenylamine was determined in triplicate. The coefficient of variation of the measure was 0. Log Koc = 3.02 L/kg

Description of key information

According to an OECD 121 GLP compliant study, log Koc of nitrosodiphenylamine is 3.02

Key value for chemical safety assessment

Koc at 20 °C:
1 047

Additional information

[LogKoc: 3.02]