p-toluic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: E & E solution Inc., Lot. No.;10206
- Expiration date of the lot/batch: no data
- Purity test date: no data

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method: The test concentrations were measured at the start and before water renewal (after 24-hour) using HPLC.
- Sample storage conditions before analysis: Stock solution was prepared by diluting the test chemical with dilution water and mixing for 24 hours or more. Test chemical was stored in desiccator (room temperature).

Vehicle:

no

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Japanese rice fish
- Strain: no data
- Source: Fish farm
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 2.0 +/- 0.5 cm
- Weight at study initiation (mean and range, SD): no data
- Method of breeding: no data
- Maintenance of the brood fish: no data

ACCLIMATION
- Acclimation period: > 12 days
- Acclimation conditions (same as test or not): no data
- Type and amount of food during acclimation: Medaka food (Kyorin Co. Ltd.
- Feeding frequency during acclimation: no data
- Health during acclimation (any mortality observed): The mortality of the test organisms for 7 days before testing was 1.5 %.

FEEDING DURING TEST
- Food type: no data
- Amount: no data
- Frequency: no data

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

26.2 as CaCO3

Test temperature:

24 +/- 1°C

pH:

6.0 - 8.5

Dissolved oxygen:

5.1 - 8.3 mg/l

Nominal and measured concentrations:

Nominal Concentrations: control, 35.0, 45.5, 59.2, 76.9 and 100.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 3 L glass vessel.
- Type (delete if not applicable): no data
- Material, size, headspace, fill volume: no data
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): n/a
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water was prepared from tap water which was dechlorinated and treated by activated carbon. Before use, the water was aerated.
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours light: 8 hours dark.
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 35.0, 45.5, 59.2, 76.9 and 100.0 mg/L
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

63.9 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

45.1 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

76.5 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Description of key information

The acute toxicity study to Oryzias latipes, the Japanese rice fish, returned a result of LC50 (96h) 63.9 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

63.9 mg/L