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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: E & E solution Inc., Lot. No.;10206
- Expiration date of the lot/batch: no data
- Purity test date: no data

Analytical monitoring:
yes
Details on sampling:
- Concentrations:
- Sampling method: The test concentrations were measured at the start and before water renewal (after 24-hour) using HPLC.
- Sample storage conditions before analysis: Stock solution was prepared by diluting the test chemical with dilution water and mixing for 24 hours or more. Test chemical was stored in desiccator (room temperature).
Vehicle:
no
Test organisms (species):
Oryzias latipes
Details on test organisms:
TEST ORGANISM
- Common name: Japanese rice fish
- Strain: no data
- Source: Fish farm
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 2.0 +/- 0.5 cm
- Weight at study initiation (mean and range, SD): no data
- Method of breeding: no data
- Maintenance of the brood fish: no data

ACCLIMATION
- Acclimation period: > 12 days
- Acclimation conditions (same as test or not): no data
- Type and amount of food during acclimation: Medaka food (Kyorin Co. Ltd.
- Feeding frequency during acclimation: no data
- Health during acclimation (any mortality observed): The mortality of the test organisms for 7 days before testing was 1.5 %.

FEEDING DURING TEST
- Food type: no data
- Amount: no data
- Frequency: no data
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
26.2 as CaCO3
Test temperature:
24 +/- 1°C
pH:
6.0 - 8.5
Dissolved oxygen:
5.1 - 8.3 mg/l
Nominal and measured concentrations:
Nominal Concentrations: control, 35.0, 45.5, 59.2, 76.9 and 100.0 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 3 L glass vessel.
- Type (delete if not applicable): no data
- Material, size, headspace, fill volume: no data
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): n/a
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water was prepared from tap water which was dechlorinated and treated by activated carbon. Before use, the water was aerated.
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours light: 8 hours dark.
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 35.0, 45.5, 59.2, 76.9 and 100.0 mg/L
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
yes
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
63.9 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
45.1 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
76.5 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
not specified

Description of key information

The acute toxicity study to Oryzias latipes, the Japanese rice fish, returned a result of LC50 (96h) 63.9 mg/l

Key value for chemical safety assessment

LC50 for freshwater fish:
63.9 mg/L

Additional information