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Ecotoxicological information

Toxicity to microorganisms

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Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1996
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: study not undertaken to recognised guideline or GLP
Guideline:
other: no data
GLP compliance:
no
Test organisms (species):
Tetrahymena pyriformis
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
27 +/- 1°C
pH:
7.35
Nominal and measured concentrations:
Nominal concentrations; 5, 10, 25, or 50 g/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 250mL glass Erlenmeyer flask.
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 50 mL medium in a 250mL glass Erlenmeyer flask.
- Aeration:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- No. of vessels per abiotic control (replicates):
- Sludge concentration (weight of dry solids per volume):
- Weight of dry solids per volume of reaction mixture per unit of time:
- Nutrients provided for bacteria:
- Nitrification inhibitor used (delete if not applicable): none / N-allylthiourea
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Particulate matter:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:
- Details on termination of incubation:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using fewer concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Key result
Duration:
48 h
Dose descriptor:
other: IGC50
Effect conc.:
210 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Validity criteria fulfilled:
not specified
Conclusions:
The toxicity to microorganisms study determined the 50% growth inhibitory concentration (IGC50) using probit analysis. IGC50 213 mg/L

Description of key information

The toxicity to microorganisms study determined the 50% growth inhibitory concentration (IGC50) using probit analysis. IGC50 213 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
213 mg/L

Additional information