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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: ATCC22662
- Source (laboratory, culture collection): Obtained from American Type Culture Collection and cultivated in aseptic culture.
- Age of inoculum (at test initiation):
- Method of cultivation: Sterile

ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
23°C
pH:
5.2 - 9.9
Nominal and measured concentrations:
Nominal Concentrations: control, 35.0, 45.5, 59.2, 76.9, and 100.0 mg/L
Measure conc. 0h; 35.2, 45.8, 59.4, 76.8, 98.4
Measure conc.; 72h; 34.6, 45.4, 59.2, 77.6, 99.6.
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL medium in a 300mL glass Erlenmeyer flask with breathable silicon cap.
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 100 mL medium in a 300mL glass Erlenmeyer flask
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: Initial Cell Number: 10,000 cells/mL
- Control end cells density:
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):

GROWTH MEDIUM
- Standard medium used: yes/no
- Detailed composition if non-standard medium was used:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Stock Solution was prepared by diluting the test chemical with OECD medium and mixing for 24 hours.
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 4000 - 5000 lux
- Salinity (for marine algae):

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]
- Chlorophyll measurement:
- Other:

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
73.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
45.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
other: LOEC
Effect conc.:
59.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Validity criteria fulfilled:
not specified
Conclusions:
Under the conditions of this study the toxicity to algae was found to be; ECr50 73.6 mg/L and NOECr 45.5 mg/L.

Description of key information

Under the conditions of this study the toxicity to algae was found to be; ECr50 73.6 mg/L and NOECr 45.5 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
73.6 mg/L
EC10 or NOEC for freshwater algae:
45.5 mg/L

Additional information