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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain/clone: no data
- Justification for species other than prescribed by test guideline:
- Source: National Institute for Environmental Studies (Japan)
- Age of parental stock (mean and range, SD):
- Feeding during test
- Food type:
- Amount:
- Frequency:

ACCLIMATION
- Acclimation period: Parental daphnids were acclimated for 2 - 4 weeks on test condition before testing.
- Acclimation conditions (same as test or not):
- Type and amount of food: Daphnids were fed with Chlorella vulgaris, 0.1 - 0.2 mg
- Feeding frequency:
- Health during acclimation (any mortality observed): During acclimatization, mortality of parental daphnia was low and resting eggs and males were not shown.

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
26.2 mg/l as CaCO3
Test temperature:
20 +/- 1°C
pH:
6.73 - 7.75
Dissolved oxygen:
8.2 - 8.6 mg/l
Nominal and measured concentrations:
Nominal Concentrations: control, 3.00, 5.10, 8.67, 14.74, 25.06, 42.60, and 72.41 mg/L
Reference substance (positive control):
yes
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
41.5 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
14 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
69.8 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
not specified
Conclusions:
The acute toxicity to invertebrates using Daphnia magna returned a result of EC50 (48h) 41.5 mg/l.

Description of key information

The acute toxicity to invertebrates using Daphnia magna returned a result of EC50 (48h) 41.5 mg/l.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
41.5 mg/L

Additional information