dinotefuran (ISO); 1-methyl-2-nitro-3-(tetrahydro-3-furylmethyl)guanidine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10/11/1999 - 20/12/1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP, Guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Sampling at 0-hour (test initiation) and 96-hour (test termination)

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Species/strain: Rainbow trout (Oncorhynchus mykiss)
Wild catch: No
Age/size: Mean body length at test initiation: 5.5 ± 0.32 cm (Mean ± SD; range: 5.1 - 5.9 cm)
Mean body wet weight at test initiation: 1.9 ± 0.31 g (Mean ± SD; range: 1.4 - 2.4 g).
Kind of food: Commercial fish diet (HOKOVIT 502, 1.2 mm, supplied by H.U. Hoffmann AG, CH-4922 Bützberg, Switzerland)
Amount of food: Not reported
Feeding frequency: Not reported
Pretreatment: The test fish were treated for diseases immediately after arriving at the test facility, then held for more than two weeks without any further medication. The fish were then acclimated for one additional week prior to the test start to the test water and temperature (12 – 13ºC).
Feeding of animals during test: No

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

1.25 mmol/L (= 125 mg/L) as CaCO3

Test temperature:

12 – 13°C

pH:

7.7 – 8.0

Dissolved oxygen:

8.9 – 9.6 mg/L

Salinity:

Not applicable

Nominal and measured concentrations:

See table below

Details on test conditions:

Test water aerated slightly until oxygen saturation was reached.
Photoperiod: 16 hours light/8 hours dark daily

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% C.I.

Details on results:

No concentration/response curves were reported.
No adverse effects.

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

None

Sublethal observations / clinical signs:

Nominal and measured concentrations:

Nominal test Concen-tration (mg/L)	Sample date     (day)	Sample Age     (hours)	Measured Concent-ration   (mg/L)^1	% of Nominal^2	Mean Measured Concentrat-ion (mg/L)	Mean Measured % of Nominal
100	0 0	0 0	98.94 99.88	99 100	99.4	99
	4 4	96 96	99.26 99.80	99 100	99.5	100

Validity criteria fulfilled:

yes

Conclusions:

No remarkable observations were made concerning the appearance of the test medium. The pH values in the test medium and the control ranged from 7.7 to 8.0. The oxygen concentration was always 8.9 mg/L or higher, and thus higher than 60% oxygen saturation. The water temperature ranged from 12 to 13 °C.

In the control and at the test concentration of 100 mg/L no mortality or other signs of intoxication were observed during the test period of 96 hours. Therefore, the 96-hour NOEC was 100 mg/L. The 96-hour LC0 was determined to be ≥ 100 mg/L and the 96-hour LOEC, the 96-hour LC50, and the 96-hour LC100 of dinotefuran to rainbow trout were all determined to be > 100 mg/L.

After 96 hours of exposure, there were no mortalities or signs of intoxication. Based on the results of this study, dinotefuran would be classified as practically nontoxic to juvenile rainbow trout (Oncorhynchus mykiss) on an acute toxicity basis.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L