dinotefuran (ISO); 1-methyl-2-nitro-3-(tetrahydro-3-furylmethyl)guanidine

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish early-life stage toxicity

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22/02/2001 - 06/12/2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP, Guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1400 (Fish Early-life Stage Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Sampling days: 0, 6, 13, 20, 27, 31, 35, 41, 48, 52, 55, 62, 69, 76, 84, and 94

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Species/strain: Rainbow trout (Oncorhynchus mykiss)
Source: Forellenhof Oristal, CH4410 Liestal, Switzerland
Wild caught: No
Age/size: Newly fertilised eggs
Kind of food: Hokovit 500 and Hokovit 502 (H.U. Hofmann AG, CH-4922 Bützberg, Switzerland)
Amount of food: Fish were fed ad libitum
Feeding frequency: Twice a day
Post-hatch transfer time: Not applicable
Time to first feeding: Before total consumption of the yolk sack
Feeding of animals during test: Yes
Treatment for disease within 2 weeks preceding test: n.a.

Test type:

flow-through

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

94 d

Post exposure observation period:

None

Hardness:

198 – 342 mg/L as CaCO3

Test temperature:

9.9 - 12.9 ºC

pH:

7.0 - 7.8

Dissolved oxygen:

8.1 - 10 mg-L

Salinity:

Not applicable

Nominal and measured concentrations:

See table below

Details on test conditions:

Intensity of irradiation: study start – one week after hatching: in the dark; one week after hatch – end of exposure: 340 – 470 Lux.
Photoperiod: 16 hour light / 8 hour dark with a 30 min transition period each

Reference substance (positive control):

no

Duration:

94 d

Dose descriptor:

NOEC

Effect conc.:

6.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

growth and survival

Details on results:

See table below.

Results with reference substance (positive control):

Not applicable

Effect	Control	10mg/L (Test concentration)	NOEC
Fertilisation rate [%]	93 – 100 %	n.d.	n.a.
Start hatch [d]	31	31	n.a.
End hatch [d]	34	34	n.a.
Hatching rate [%]	92	97	≥ 10 mg/L
Development rate [1/d]	0.031	0.031	≥ 10 mg/L
Swim up [%]	98	97	≥ 10 mg/L
Mortality [n]	1	2	≥ 10 mg/L
Visible abnormalities[n]	0	0	≥ 10 mg/L
Length[mm]	41.0 ± 5.3	41.9 ± 4.5	≥ 10 mg/L
Dry weight[g]	0.15 ± 0.05	0.16 ± 0.06	≥ 10 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The test item concentration on the sampling days 48, 69, 64 and 65 ranged from 64-75 % and ranged from 109 to 129 % of the nominal concentration at any other sampling day, resulting in an overall mean measured test concentration of 104 %. There was no indication for malfunction of the dosing system, the water flow or the consumption of the application solution.

Since no adverse effects were observed throughout the course of the study, using the lowest measured test item concentration would be a worst-case scenario still leading to a robust endpoint. Therefore the author of this summary recommends to use an overall NOEC of 6.4 mg/L rather than the reported overall NOEC of 10 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

10.1 mg/L