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Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Exposure: 26. February 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study (GLP)
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Qualifier:
according to
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Qualifier:
according to
Guideline:
other: EC guideline 88/302/EEC
GLP compliance:
yes
Specific details on test material used for the study:
Batch number Vers. 98/044-10
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Source: Activated sludge from laboratory wastewater plants treating municipal and synthetic sewage.
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/I dry substance and aerated for 24 hours .
50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test .
Test type:
static
Water media type:
freshwater
Total exposure duration:
30 min
Test temperature:
20 ±2°C
pH:
7.4
Nominal and measured concentrations:
Nominal test concentration: 1000 mg/L
Details on test conditions:
TEST CONCENTRATIONS
- Test concentrations: 1000 mg/L

TEST SYSTEM
- Test vessel: Erlenmeyer glass vessel (nominal value 250 mL)
- Fill volume: 250 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC50 of 3,5 -dichlorophenol in the range of 5-30 mg/I: yes
Reported statistics and error estimates:
Graphical interpreation of the results.

Summary of measurements:

Vessel no.:

BC 1

BC 2

BC 3

mvBC

TS 1

Concentration of test substance (mg/L)

-

-

-

-

1000

Added volume of stock solution (mL/vessel)

-

-

-

-

125.5

Direct addition (mg/vessel)

-

-

-

-

-

Oxygen concentration start (mg O2/L)

8.3

8.2

8.0

-

8.4

Oxygen concentration after 6 min (mg O2/L)

5.7

5.8

5.5

-

6.0

Oxygen consumption rate (mg O2/L*6 min)

2.6

2.4

2.5

2.5

2.4

Oxygen consumption rate (mg O2/L*h)

26

24

25

25

24

Specific oxygen consumption rate (mg O2/g*h)

26

24

25

25

24

Change in oxygen consumption rate

compared with the blank control (%)

-

-

-

-

4

- BC: blank control

- mvBC: mean value of blank control

- TS: test substance

 

pH values – blank control (BC) and test substance (TS):

Vessel no.:

BC 1

BC 2

BC 3

TS 1

pH-value before adding the inoculum

before correction:

after correction:

 

6.7

7.2

 

6.7

7.2

 

6.7

7.3

 

9.5

7.4

pH-value after an incubation time of 30 min

-

-

-

7.6

Validity criteria fulfilled:
yes
Conclusions:
Based on the results of oxygen consumption, the EC50 (30 minutes) for 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0]was estimated to be >1000 mg/L.
Executive summary:

The toxicity to bacteria of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] (identified as Halb-PMDETA in the study report) was investigated according to OECD 209. Activated sludge (mixed population of different micro-organisms) was exposed for 30 minutes to a nominal concentration of 1000 mg/L. Two controls without test substance are included in the test design. 3,5-dichlorophenol was used as a reference substance.

Based on the results of oxygen consumption, the EC50 (30 minutes) for 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] was estimated to be >1000 mg/L, indicating low toxicity of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] to aerobic waste water treatment bacteria.

Description of key information

The EC50 (30 minutes) for 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0]was estimated to be >1000 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information

The toxicity to bacteria of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] (identified as Halb-PMDETA in the study report) was investigated according to OECD 209. Activated sludge (mixed population of different micro-organisms) was exposed for 30 minutes to a nominal concentration of 1000mg/L. Two controls without test substance are included in the test design. 3,5-dichlorophenol was used as a reference substance.

Based on the results of oxygen consumption, the EC50 (30 minutes) for 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0]was estimated to be >1000 mg/L, indicating low toxicity of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0]to aerobic waste water bacteria.