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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Exposure: 03. - 05. March 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study (GLP)
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Qualifier:
according to
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
GLP compliance:
yes
Specific details on test material used for the study:
Batch number Vers. 98/044-10
Analytical monitoring:
yes
Details on sampling:
Samples were taken from the following nominal concentrations:
- 100 mg/L
- 12.5 mg/L

At test beginning (after 0 h) samples from vessels without daphnids were taken; at test end (after 48 h) samples from vessels with and without daphnids were taken.

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was stirred in M4 medium for about 15 minutes at 20 ± 2°C. The nominal concentration of the stock solution was 100 mg/L . By dilutiñg this stock solution with M4 medium the nominal concentrations prepared.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain: Daphnia magna STRAUS
- Source: In-house laboratory culture
- Clone supplier: Institut National de Recherche Chimique Appliquée, France (supplied in 1978).
- Age at study initiation (mean and range, SD): 2 to 24 hours (starting with the 3rd breed of parent animals)



Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.42 mmol/L
Test temperature:
20.2 - 20.6 °C
pH:
8.1 - 9.5
Dissolved oxygen:
8.4 - 8.8 mg/L
Salinity:
Not applicable
Conductivity:
601 uS/cm
Nominal and measured concentrations:
Nominal test concentrations: 0 (control), 12.5, 25, 50 and 100 mg/L
Mean measured concentrations; <5 (control), 13 and 102 mg/L.


Details on test conditions:
TEST SYSTEM
- Test vessel: Glass tubes with flat bottom (20 mL)
- Fill volume: 10 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of parallel vessels without animals: 1 per test concentration and control for measurement of the pH and oxygen content at the beginning of the test. 2 per test concentration and control for concentration control analysis.

TEST MEDIUM / WATER PARAMETERS
Elendt M4 medium:
- Total hardness: 2.2 - 3.2 mmol/L
- Alkalinity up to pH 4.3: 0.8 - 1 mmol/L
- Molar ratio (Ca : Mg): about 4 : 1
- pH value: 7.5 - 8.5
- Conductivity: 550 - 650 µS/cm
After preparation the M4 medium is aerated for approximately 24 h until saturation with oxygen is reached .

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: L:D = 16 :8 (e.g. OSRAM L58 W31)
- Light intensity: about 2 - 7 µE/(m^2*s) ( wave length 400 - 700 nm)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Immobilization

TEST CONCENTRATIONS
- Test concentrations: 0 (control), 12.5, 25, 50 and 100 mg/L
- Range finding study: yes; in a preliminary investigation the neutralized and the not neutralized samples showed no effect on the
swimming ability of Daphnia magna up to 100mg/L .
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: nominal, confirmed by measurements (detected test item within +/-20% of nominal concentration)
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: nominal, confirmed by measurements (detected test item within +/-20% of nominal concentration)
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: nominal, confirmed by measurements (detected test item within +/-20% of nominal concentration)
Results with reference substance (positive control):
The 24 EC50 of potassium dichromate was 1.26 mg/L, experiment conducted on 15 February 1999.
Reported statistics and error estimates:
Endpoints were determined by observation of the data.

Number of mobile daphnids (total of 4 replicates):

Concentration

[mg/L]

mobile Daphnids after

3 h

6 h

24 h

48 h

0 (control)

20

20

20

20

12.5

20

20

20

20

25

20

20

20

20

50

20

20

20

20

100

20

20

20

17

 

Analytical results

Nominal concentration (mg/L)

Measured concentration (mg/L)*

Mean measured concentrations (mg/L)

0 hours

48 hours

Control

<5

<5, <5

<5

12.5

13

12, 13

13

100

108

99, 100

102

Each reported value is a mean of two determinations

 

pH- and oxygen values (after 0 and 48 h):

Concentration [mg/L]

pH after

O2 after

0 h

48 h

0 h

48 h

0 (control)

8.2

8.1

8.8

8.5

12.5

8.7

8.1

8.6

8.4

25

9.0

8.2

8.6

8.4

50

9.3

8.4

8.6

8.4

100

9.5

8.7

8.6

8.6

Validity criteria fulfilled:
yes
Conclusions:
The effects of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] to Daphnia magna were determined in a 48 hour static study. The 48 hour EC50 was >100 mg/L and the corresponding NOEC was observed to be 100mg/L.
Executive summary:

The effects of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] to Daphnia magna were determined in a 48 hour static study. The study was carried out according to OECD guideline 202 (1984). In this study Daphnia were exposed to the test material over a test period of 48 hours and were exposed to nominal exposure concentrations of 0 (control), 12.5, 25, 50 and 100 mg/L. Analysis of the 12.5 and 100 mg/L test media at the start and end of the exposure periods provided mean measured exposure concentrations of 13 and 102 mg/L demonstrating that the test material was stable for the duration of the exposure period.  The results of the study were therefore based on nominal exposure concentrations. After 48 hours there was no immobile daphnids in the control, 12.5, 25 and 50 mg/L treatments and 15% immobility in the 100 mg/L treatment. The 48 hour EC50 was therefore observed to be >100 mg/L and the corresponding NOEC was observed to be 100 mg/L.

Description of key information

The 48 hour EC50 of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] to Daphnia magna was >100 mg/L and the corresponding NOEC was observed to be 100mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

The effects of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] to Daphnia magna were determined in a 48 hour static study. The study was carried out according to OECD guideline 202 (1984). In this study Daphnia were exposed to the test material over a test period of 48 hours and were exposed to nominal exposure concentrations of 0 (control), 12.5, 25, 50 and 100 mg/L. Analysis of the 12.5 and 100 mg/L test media at the start and end of the exposure periods provided mean measured exposure concentrations of 13 and 102 mg/L demonstrating that the test material was stable for the duration of the exposure period.  The results of the study were therefore based on nominal exposure concentrations. After 48 hours there was no immobile daphnids in the control, 12.5, 25 and 50 mg/L treatments and 15% immobility in the 100 mg/L treatment. The 48 hour EC50 was therefore observed to be >100 mg/L and the corresponding NOEC was observed to be 100mg/L.