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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20.03. - 17.04.1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Qualifier:
equivalent or similar to
Guideline:
ISO 7827 (Evaluation in an Aqueous Medium of the "Ultimate" Aerobic Biodegradability of Organic Compounds - Method by Analysis of Dissolved Organic Carbon (DOC))
Qualifier:
according to
Guideline:
other: EG-guideline 92/69/EWG
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from laboratory wastewater plants treating municipal and synthetic sewage
Duration of test (contact time):
28 d
Initial conc.:
20 mg/L
Based on:
DOC
Initial conc.:
35 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST SYSTEM
- Test vessel: 1 L-glass bottle
- Fill volume: 1000ml
- Aeration: yes
- No. of vessels test substance (replicates): 2
- No. of vessels blank control (replicates): 2
- No. of vessels reference substance (replicates): 1

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Test period: 28 days
- Incubation temperature: room temperature (approx. 20°C)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
DOC removal
Reference substance:
aniline
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
0 d
Parameter:
% degradation (DOC removal)
Value:
5
Sampling time:
3 d
Parameter:
% degradation (DOC removal)
Value:
1
Sampling time:
7 d
Parameter:
% degradation (DOC removal)
Value:
8
Sampling time:
14 d
Parameter:
% degradation (DOC removal)
Value:
1
Sampling time:
28 d
Results with reference substance:
Degradation of the reference substance anilline was 95% after 5 days.

Summary of results – DOC decrease [%]:

Test duration [days]

Reference substance

Inhibition control

Adsorption control

TS 1

TS 2

TS mean value

0

0

0

0

0

0

0

1

2

-1

-7

-9

-3

-6

3

7

2

2

3

6

5

5

95

44

2

1

7

4

7

95

45

n.p.

-1

2

1

10

91

47

n.p.

-4

0

-2

14

94

46

n.p.

4

11

8

17

93

43

n.p.

-1

3

1

21

95

43

n.p.

-14

-6

-10

24

88

45

n.p.

-3

0

-2

27

96

43

n.p.

-8

-1

-5

28

95

46

n.p.

1

1

1

TS: Test substance

n.p.: no adsorption control performed

 

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Not readily biodegradable (according to OECD criteria)
Executive summary:

A reliable, GLP compliant experimental aerobic ready biodegradability study was conducted according to EG-guideline 92/69/EWG (equivalent to the OECD 301A Guideline (ready biodegradability: DOC Die Away Test) and ISO 7827 method). There were no deviations from the test guideline and the validity criteria for testing of ready biodegradability according to OECD criteria were fulfilled.

The reference substance aniline showed 95% degradation (DOC removal) after 5 days, however only 1% degradation (DOC removal) of the test substance, 2-[[2-(dimethylamino)ethyl]methylamino]ethanol (CAS 2212-32-0), was shown after 28 days. It is therefore concluded that 2-[[2-(dimethylamino)ethyl]methylamino]ethanol is not readily biodegradable.  

Description of key information

A reliable, GLP compliant experimental aerobic ready biodegradability study was conducted according to EG-guideline 92/69/EWG (equivalent to the OECD 301A Guideline (ready biodegradability: DOC Die Away Test) and ISO 7827 method). There were no deviations from the test guideline and the validity criteria for testing of ready biodegradability according to OECD criteria were fulfilled. The reference substance aniline showed 95% degradation (DOC removal) after 5 days, however only 1% degradation (DOC removal) of the test substance, 2-[[2-(dimethylamino)ethyl]methylamino]ethanol (CAS 2212-32-0), was shown after 28 days.

It is therefore concluded that 2-[[2-(dimethylamino)ethyl]methylamino]ethanol is not readily biodegradable.  

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information