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EC number: 233-251-1 | CAS number: 10101-50-5
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- See the read-across report attached in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.17 mg/L
- Conc. based on:
- other: Mn
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 30 Hardness. 95 % CL 2.90 - 3.47 mg/L.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 16.2 mg/L
- Conc. based on:
- other: Mn
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 150 Hardness.95 % CL 14.0 - 18.7 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- See the read-across report attached in Section 13.
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Acute toxicity tests performed on fry as part of a fish early life stage test
- GLP compliance:
- not specified
- Details on sampling:
- - Concentrations: All
- Sample storage conditions before analysis: Preserved with Ultrex, triple distilled nitric acid - Vehicle:
- no
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: Colorado Division of Wildlife Crystal River Hatchery brood stock - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Performed as part of a 33 day fish early life stage study
- Hardness:
- 30 hardness
29.2 mg/L as CaCO3
150 hardness
151.2 mg/L as CaCO3 - Test temperature:
- 30 hardness
12.4°C
150 hardness
11.6°C - pH:
- 30 hardness
7.52
150 hardness
7.61 - Dissolved oxygen:
- 30 hardness
8.9 mg/L
150 hardness
8.8 mg/L - Nominal and measured concentrations:
- 30 hardness
Measured mg/L
<0.02, 0.42, 0.76, 1.47, 2.85, 5.14, 8.40, 18.7
150 hardness
Measured mg/L
<0.02, 1.25, 2.08, 3.39, 6.48, 18.3, 37.7, 77.2 - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria containing approximately 2.2 L
- Biomass loading rate:5g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
30 mg/L hardness: Dechlorinated Fort Collins municipal water and water deionized by reverse osmosis
150 mg/L hardness: Dechlorinated Fort Collins municipal water and well water from an on site well
OTHER TEST CONDITIONS
- Photoperiod: 16h light/8h dark
- Light intensity: 107.5 - 193.75 Lux - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.17 mg/L
- Conc. based on:
- other: Mn
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 30 Hardness. 95%CL 2.90 - 3.47
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 16.2 mg/L
- Conc. based on:
- other: Mn
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 150 Hardness. 95% CL 14.0 - 18.7
- Sublethal observations / clinical signs:
96 hour median lethal concentrations (LC50) for rainbow trout fry were 3.17 and 16.2 mg Mn/L in water hardness of 30 and 150 respectively.
Table 1: water quality characteristics
Statistics
Hardness (as CaCO3) mg/L
Alkalinity (as CaCO3) mg/L
pH
Dissolved oxygen mg/L
Temperature °C
Conductivity µS/c,
30 Hardness
Mean
27.6
20.4
7.45
6.8
13.3
49.9
Range
25.4 – 29.8
19.0 – 22.4
7.22–2.76
5.3 – 8.1
12.2 – 14.3
43.8 – 57.9
150 Hardness
Mean
147.8
104.6
7.52
6.7
12.6
252
Range
132.2 – 156.4
92.2 – 113.8
7.48-7.62
5.1 – 8.3
12.0 – 13.3
220 - 268
Table 2: Summary of anions for laboratory dilution waters
Parameter
mg/L
30 Hardness
150 Hardness
Mean
Range
Mean
Range
Fluoride
0.65
0.20 – 0.80
0.60
0.50 – 0.90
Chloride
1.42
0.80 – 1.80
6.75
2.00 – 8.20
Nitrate
0.94
0.50 – 5.40
6.16
0.70 – 7.20
Phosphate
<0.5
- -
<0.5
- -
Sulphate*
7.0
7.0 – 7.1
55.0
52.0 – 59.6
*Data represents measurements from control tank only
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the study the lowest LC50 was 3.2 mg Mn/L.
- Executive summary:
The short-term toxicity of the test material to fish was performed on fry as part of a fish early life stage test.
Under the conditions of the study the lowest LC50 was 3.2 mg Mn/L.
Referenceopen allclose all
Description of key information
Under the conditions of the study the lowest LC50 for the read across source substance was 3.2 mg Mn/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 3.2 mg/L
Additional information
The short-term toxicity of the source substance to fish was performed on fry as part of a fish early life stage test. The study was awarded a reliability score of 2 in accordance with the criteria set forth by Klimisch et al. (1997).
Under the conditions of the study the lowest LC50 was 3.2 mg Mn/L.
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