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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard OECD test guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
This study was designed to assess the toxic effects of the test compound on the Zebra fish(Danio rerio) .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 200 mg of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L ,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Source: Koyal Aquaruim, Nagpur
- Length at study initiation (length definition, mean, range and SD): average 1.73 cm
- Weight at study initiation (mean and range, SD): 0.422g (average)
- Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Duration of housing: 15 days
- Method of breeding: No data
- Maintenance of the brood fish:No data

ACCLIMATION
- Acclimation period:7 days
- Acclimation conditions (same as test or not): Photoperiod:16 hours light and 8 hours dark, Temperature range: 24.5 °C, pH range: 7.65, DO range: 7.7 mg/l.
- Type and amount of food during acclimation:Standard brand feed
- Feeding frequency during acclimation:Daily once
- Health during acclimation (any mortality observed): During last 48 hours = 0

FEEDING DURING TEST
- Food type:Standard brand feed
- Frequency:once daily
- Withdrawal of food: 1 day before and during exposure
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
150.5 mg of CaCO3
Test temperature:
23 °C
pH:
7.05
Dissolved oxygen:
7.7 mg/l
Salinity:
No data
Conductivity:
No data
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel:The test vessels used were all plastic aquaria having the water capacity of 5liters.
- Material, size, headspace, fill volume: 2 liters
- Aeration: Continous
Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel:8 fishes
- No. of vessels per concentration (replicates): No data
- No. of vessels per control (replicates): No data
- No. of vessels per vehicle control (replicates): No data
- Biomass loading rate: No data

OTHER TEST CONDITIONS
- Adjustment of pH:No
- Photoperiod:16 hours light and 8 hours dark
- Light intensity: No data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :Determination or calculation of the median lethal concentrations (LC50) and observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24, 48, 72 and 96 hours of the start of the experiment.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/l





Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: No effect was observed on the test animal
Details on results:
A 96 h study was conducted for test material on Zebra fish(Danio rerio), at a test concentrations of 100mg/L . The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria.
The lethal concentrations LC50 was found to be > 100 mg/L
LC0 (96 hours) (highest loading at which no mortality was observed) =100 mg/L
LC50 (96 hours) Experimental = > 100 mg/L

Mortality and symptoms

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

 

3 h

6 h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

-

-

-

-

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Symptom (cumulated) after

 

3 h

6 h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

Nominal

Concentration

(mg/l)

Number of

fish

pH readings after

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

8.12

8.15

8.23

8.25

8.31

8.32

8.32

8.31

100

4

4

7.89

7.86

8.01

8.05

8.24

8.26

8.25

8.26

 

Nominal

Concentration

(mg/l)

Number of

fish

Oxygen content (mg/L) after

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.0

7.0

6.9

6.9

6.9

6.9

6.8

6.8

100

4

4

 7.0

7.0

6.9

6.9

6.8

6.8

6.7

6.7

 

Test temperature

Nominal

Concentration

(mg/l)

Number of

fish

Temperature (°C) after

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

22.0

22.0

23.2

23.2

23.5

23.5

23.6

23.6

6.25

4

4

22.0

22.0

23.5

23.5

23.5

23.5

23.6

23.6

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test substance on Zebra fish(Danio rerio)was found to be > 100 mg/L.
Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test substance to assess the toxic effects of the test compound on the Zebra fish(Danio rerio). Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study. The nominal concentration selected for the experiment were 100 mg/Land test fish were exposed to these concentration for 96 hours. The median lethal concentrations LC50 for test material on Danio rerio in a 96 hours study on the basis of mortality effect was found to be > 100 mg/L. Thus, on the basis of this LC50 value and according to CLP criteria for aquatic classification of the substa nce, it is concluded that the test substance, does not exhibit short term toxicity to fish. LC0 (96 hours) (highest loading at which no mortality was observed) =100 mg/L . Hence , based on the above effect concentrationit can be concluded that test material has no effect on aquatic invertebrates and can not be classified as per CLP criteria.

Description of key information

Short-term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test substance to assess the toxic effects of the test compound on the Zebra fish(Danio rerio). Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study. The nominal concentration selected for the experiment were 100 mg/Land test fish were exposed to these concentration for 96 hours. The median lethal concentrations LC50 for test material on Danio rerio in a 96 hours study on the basis of mortality effect was found to be > 100 mg/L. Thus, on the basis of this LC50 value and according to CLP criteria for aquatic classification of the substa nce, it is concluded that the test substance, does not exhibit short term toxicity to fish. LC0 (96 hours) (highest loading at which no mortality was observed) =100 mg/L . Hence , based on the above effect concentrationit can be concluded that test material has no effect on aquatic invertebrates and can not be classified as per CLP criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

Short-term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test substance to assess the toxic effects of the test compound on the Zebra fish(Danio rerio). Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study. The nominal concentration selected for the experiment were 100 mg/Land test fish were exposed to these concentration for 96 hours. The median lethal concentrations LC50 for test material on Danio rerio in a 96 hours study on the basis of mortality effect was found to be > 100 mg/L. Thus, on the basis of this LC50 value and according to CLP criteria for aquatic classification of the substa nce, it is concluded that the test substance, does not exhibit short term toxicity to fish. LC0 (96 hours) (highest loading at which no mortality was observed) =100 mg/L . Hence , based on the above effect concentrationit can be concluded that test material has no effect on aquatic invertebrates and can not be classified as per CLP criteria.