6-aminonaphthalene-2-sulphonic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result using OECD guidelines.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the test Daphnids.Beaker containing 20ml of media with 10 Daphinds. A semi-static procedure was used for the study and it was conducted in compliance with the OECD guideline 202.

GLP compliance:

no

Analytical monitoring:

no

Details on sampling:

No data available

Vehicle:

yes

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 50 mg of the test substance in 500 ml of ADaM’s media. Achieving test concentrations of 100 mg/L, respectively.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: No data available
- Source: Own breeding of Daphnia magna
- Age: No data available
- Feeding during test : No feeding after hatching
- Food type: No data available
- Amount: No data available
- Frequency: No data available

ACCLIMATION
- Acclimation period: No data available
- Acclimation conditions (same as test or not): No data available
- Type and amount of food: No data available
- Feeding frequency: No data available
- Health during acclimation (any mortality observed): No data available

QUARANTINE (wild caught)
- Duration: No data available
- Health/mortality: No data available

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

No data available

Hardness:

150.5 mg of CaCO3

Test temperature:

20 °C ± 2 °C

pH:

7.15

Dissolved oxygen:

No data available

Salinity:

No data available

Conductivity:

No data available

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: The test vessels used were all glass beaker having the water capacity of 25ml.
- Material, size, headspace, fill volume: 20 ml
- Aeration: No aeration during experiment
- No. of organisms per vessel: 10 Daphnids
- No. of vessels per concentration (replicates): No data available
- No. of vessels per control (replicates): No data available
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductivity: No data available
- Salinity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available

OTHER TEST CONDITIONS
- Adjustment of pH: No data available
- Photoperiod: 16 hours light and 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Determination or calculation of the median effective concen trations (EC 50) and observations ((immobility, pH, Temperature, dissolved oxygen content) ) of the symptoms were done after 24 and 48 hours.

VALIDITY OF THE TEST:
1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobiliz ed.
2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

A 48 h study was conducted for test material on test daphnia, at a test concentrations of 100 mg/L. The daphnids were moving slowly as compared to control.No Immobility were found in the control beaker. EC50 (48 hours) Experimental = >100 mg/L

OBSERVATIONS:

Immobility

Nominal Concentration (mg/l)	Number of Daphnids	Immobility
		0 h	24 h	48 h
Control	10	-	-	-
100	10	-	-	-

 

pH and oxygen content:

Nominal Concentration (mg/l)	Number of daphnids	pH(mg/L)
		0h	48h
Control	10	7.13	7.17
100	10	7.15	7.20

 

Nominal Concentration (mg/l)	Number of daphnids	DO(mg/L)
		0h	48h
Control	10	7.05	6.99
100	10	7.01	6.95

 

Test temperature

Nominal Concentration (mg/l)	Number of daphnids	Test temperature
		0h	48h
Control	10	20.5	20.1
100	10	20.5	20.1

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test material on test daphnia was found to be >100 mg/L.

Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 202 was conducted for test material to assess the toxic effects of the test compound on the test Daphnids. Beaker containing 20ml of media with 10 Daphinds. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 50 mg of the test substance in 500 ml of ADaM’s media. Achieving test concentrations of 100 mg/L, respectively.

A semi-static procedure was used for the study. The nominal concentration selected for the experiment was 100 mg/L and test Daphnids were exposed to this concentration for 48hours. The Effective concentrations EC50 was found to be >100 mg/L .

Thus, according to the CLP Criteria for aquatic classification of the substance, it is concluded that test material does not exhibit toxicity to aquatic invertebrate (daphnia magna) and therefore it cannot be classified as hazardous substance.

Description of key information

Short-term toxicity to aquatic invertebrates:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 202 was conducted for test material to assess the toxic effects of the test compound on the test Daphnids. Beaker containing 20ml of media with 10 Daphinds. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 50 mg of the test substance in 500 ml of ADaM’s media. Achieving test concentrations of 100 mg/L, respectively.

A semi-static procedure was used for the study. The nominal concentration selected for the experiment was 100 mg/L and test Daphnids were exposed to this concentration for 48hours. The Effective concentrations EC50 was found to be >100 mg/L.

Thus, according to the CLP Criteria for aquatic classification of thesubstance, it is concluded that test material does not exhibit toxicity to aquatic invertebrate (daphnia magna) andtherefore it cannot be classified as hazardous substance.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Short-term toxicity to aquatic invertebrates:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 202 was conducted for test material to assess the toxic effects of the test compound on the test Daphnids. Beaker containing 20ml of media with 10 Daphinds. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 50 mg of the test substance in 500 ml of ADaM’s media. Achieving test concentrations of 100 mg/L, respectively.

A semi-static procedure was used for the study. The nominal concentration selected for the experiment was 100 mg/L and test Daphnids were exposed to this concentration for 48hours. The Effective concentrations EC50 was found to be >100 mg/L.

Thus, according to the CLP Criteria for aquatic classification of thesubstance, it is concluded that test material does not exhibit toxicity to aquatic invertebrate (daphnia magna) andtherefore it cannot be classified as hazardous substance.