2,4-diaminobenzenesulphonic acid

Endpoint summary

Additional information

Short-term toxicity to fish:

Predicted model data and experimental study result for the test chemical 2,4-Diaminobenzenesulfonic acid (CAS No. 88-63-1) with respective read across were reviewed to summarize the following information:

In key study the 96 hrs aquatic fish toxicity study was predicted to assess toxic effects of the test compound 2,4-Diaminobenzenesulfonic acid (CAS No. 88-63-1)and the result were predicted (SSS QSAR Prediction model, 2016). The study was based on the effects of the test compound on Pimephales promelas fish in a static fresh water system. The predicted data suggests the lethal concentration (LC50) for the test compound was estimated to be 274.723480225 mg/l on the basis of mortality effect.

Whereas supporting model which is based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10 (EPI Suite, 2016), the 96 hours LC50 was estimated to be 14900000 mg/l on fish for test substance 2,4-Diaminobenzenesulfonic acid (CAS No. 88-63-1)with mortality effects.

Based on above predicted value from SSS QSAR and ECOSAR, it can be concluded that the substance can be considered as non toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Above predicted lethal concentrations supported by experimental study (Chemosphere. Vol. 28, No. 12, pp. 2203.2236, 1994)for read across chemical Sulfanilic acid (Cas no. 121-57-3) which is indicates that Toxic effects of test substance 4-amino-benzenesulfonic acid was determined on various fish species like Leuciscus idus, Bruchydunio rerio,Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate for 48 and 96 hrs in static condition and in fresh water.

During experiment the lethal concentrations i.e LC0 and LC50 values were found to be 1000 mg/l and 100.4 mg/l respectively.

Different experimental data (J. Hydrology; 51:359-367; 1981) for same read across i.e Sulfanilic acid (Cas no. 121-57-3) was indicate toxic effects of test chemical Sulfanilic acid on fish (Pimephales promelas) in static fresh water with study condition of 40-48 mg/L CaCO3 hardness,22±1°C test temp.,7.2-7.9 pH,> 40 % Sat dissolved oxygen and 120 to 160 uS/cm conductivity.

During study the lethal concentration (LC50) was found to be 100.4 mg/l on the basis of mortality effect.

Another read across 3-methylsulphanilic acid (Cas no. 98-33-9) from experimental study indicate that the acute toxicity to fish test been performed to evaluate the toxicological effects of chemical 4-Amino-m-toluenesulfonic acid in fish. The chemical was exposed to Gambusia affinis fish with dose concentration of 180, 320, 560, 1000 mg/L with exposure duration of 24, 48 and 96 hrs.The mortality was observed in the fish, at 560 and 1000 mg/L all animals were dead in 6 hrs only.Hence, the LC50 value was found to be 375 mg/L, 425 mg/L and 410mg/L when with duration of 96, 24 and 48-hrs respectively when tested in Gambusia affinis fish based on mortality. (Sewage and Industrial Wastes Vol. 29, No. 6 (Jun., 1957), pp. 695-711)

Whereas authorative database (Data bank of Environmental Properties of Chemicals (EnviChem), 2016) for read across 4-aminotoluene-3-sulphonic acid (Cas no. 88-44-8) indicate that toxic effects was examined on fish Oryzias latipes for 48 hrs.

During experiment the lethal concentration (LC50) was found to be 480 mg/l on the basis of mortality effects.

Thus, based on above all lethal concentration values, it can be concluded that the target chemical 2,4-Diaminobenzenesulfonic acid (CAS No. 88-63-1) can be considered as non-toxic to fish and thus cannot be classified as hazardous as per the CLP criteria.

Short term toxicity to aquatic invertebrates

Aim of this study was to assess the short term toxicity of2,4-Diaminobenzenesulfonic acidto aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs.

 

The stock solution 100 mg/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical).. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The median effective concentration (EC50) for the test substance2,4-Diaminobenzenesulfonic acid, in Daphnia magna was determined to be 62.2 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be be classified as aquatic chronic 3

Toxicity to aquatic algae and cyanobacteria

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The test solution 100 mg/l was prepared by dissolving yellow powder in OECD growth medium. Effects on the growth rate of the organism were studied (Changable). Various concentration were used.

With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (EC50) for the test substance2,4-Diaminobenzenesulfonic aci

, in algae was determined to be 110 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as per the CLP criteria.

Based on the overall reported results for target and read across substance, it can be concluded that the substance 2,4-Diaminobenzenesulfonic acid can be considered as likely hazardous to aquatic organisms and thus can be classified aquatic chronic 3 as hazardous as per the CLP criteria.