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Administrative data

Description of key information

2,4-diaminobenzenesulphonic acid was noon toxic by oral route in rats

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records
Reference
Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from authorative handbook
Qualifier:
according to
Guideline:
other: refer below principle
Principles of method if other than guideline:
Acute oral toxicity study of 1,3-Phenylenediamine-4-sulfonic acid in rat.
GLP compliance:
not specified
Test type:
other: No data available
Limit test:
yes
Species:
rat
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
No data available
Route of administration:
oral: unspecified
Vehicle:
not specified
Doses:
3480 mg/kg
No. of animals per sex per dose:
No data available
Control animals:
not specified
Details on study design:
No data available
Statistics:
No data available
Preliminary study:
No data available
Sex:
not specified
Dose descriptor:
LD50
Effect level:
3 480 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50 % mortality observed
Mortality:
No data available
Clinical signs:
No data available
Body weight:
No data available
Gross pathology:
No data available
Other findings:
No data available
Interpretation of results:
not classified
Remarks:
Migrated informationCriteria used for interpretation of results: EU
Conclusions:
Acute oral LD50 value of 1,3-Phenylenediamine-4-sulfonic acid is considered to be 3480 mg/kg in rat.
Executive summary:

Acute oral toxicity test of 1,3-Phenylenediamine-4-sulfonic acid was conducted with rat in the concentration of 3480 mg/kg mg/kg bw orally. 50 % mortality was observed in treated rat. Therefore, LD50 was considered to be 3480 mg/kg when rats were treated with 2,4-diaminobenzenesulphonic acid orally.

 

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
3 480 mg/kg bw
Quality of whole database:
Data is Klimisch 2 and from Sax’ handbook

Acute toxicity: via inhalation route

Endpoint conclusion
Endpoint conclusion:
no study available

Acute toxicity: via dermal route

Endpoint conclusion
Endpoint conclusion:
no study available

Additional information

Acute oral toxicity:

Based on the data available for target 2,4-diaminobenzenesulphonic acid (CAS no 88-63-1) and its read across 3-Aminobenzenesulfonic acid (CAS no 121-47-1) and 2- Amino-5-Methylbenzenesulfonic Acid (CAS no 88-44-8) are summarized below

 In a study given by Lewiset al(2012) in Sax’ handbook, acute oral toxicity was mentioned for rat by using 2,4-diaminobenzenesulphonic acid in the concentration of 3480 mg/kg mg/kg bw orally. 50 % mortality was observed in treated rat. Therefore, LD50 was considered to be 3480 mg/kg when rats were treated with 2,4-diaminobenzenesulphonic acid orally.

Based on the prediction done by using Danish (Q) SAR Database (2016), acute oral toxicity was estimated in rats treated with 2,4-diaminobenzenesulphonic acid in the concentration of 5000 mg/kg bw orally. 50 % mortality was observed in treated rats. Therefore, Estimated LD50 was found to be 5000 mg/kg bw when rat were treated with 2, 4-diaminobenzenesulphonic acid orally.   

In a study given by Ministry of Health J-CHECK (2016) for read across, acute oral toxicity was evaluated in Crj:CD (SD) male and female rats by using 3-Aminobenzenesulfonic acid in the concentration of 500, 1000, 2000 mg/kg orally. No effect on survival and body weight were observed in treated rats. Diarrhea with soft feces on the administration day and the following day, and yellow-discolored urine on the administration day were observed at 500 and 1000 mg/kg treated male and female rats. In addition, no gross pathological and histopathological changes were observed in treated male and female rats. Therefore, LD50 was considered to be > 2000 mg/kg bw when Crj:CD (SD) male and female rat were treated with 3-Aminobenzenesulfonic acid orally.  

In the above similar source for anther read across, acute oral toxicity was evaluated in Crj:CD (SD) male and female rats by using2- Amino-5-Methylbenzenesulfonic Acid in the concentration 12000 mg/kg orally. No effect on survival were observed in treated rat at 12000 mg/kg bw Therefore, LD50 was considered to be 12000 mg/kg bw when Crj:CD (SD) male and female rat were treated with 2- Amino-5-Methylbenzenesulfonic Acid orally.  

Thus, based on weight of evidence for for target 2,4-diaminobenzenesulphonic acid (CAS no 88-63-1) and its read across 3-Aminobenzenesulfonic acid (CAS no 121-47-1) and 2- Amino-5-Methylbenzenesulfonic Acid (CAS no 88-44-8) is likely to be non hazardous by oral route in rats.

Justification for selection of acute toxicity – oral endpoint

LD50 was considered to be 3480 mg/kg when rats were treated with 2,4-diaminobenzenesulphonic acid orally.

Justification for classification or non-classification

Based on weight of evidence for for target 2,4-diaminobenzenesulphonic acid (CAS no 88-63-1) and its read across 3-Aminobenzenesulfonic acid (CAS no 121-47-1) and 2- Amino-5-Methylbenzenesulfonic Acid (CAS no 88-44-8) is likely to be non hazardous by oral route in rats.