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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound 2,4-Diaminobenzene sulfonic acid (CAS no. 88-63-1) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that 2,4-Diaminobenzenesulfonic acid is not expected to be readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The predicted data for the test compound 2,4-Diaminobenzenesulfonic acid (CAS No. 88-63-1) and the study for its read across substance were reviewed for the biodegradation end point which are summarized as below:

Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound 2,4-Diaminobenzene sulfonic acid (CAS no. 88-63-1) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that 2,4-Diaminobenzenesulfonic acid is not expected to be readily biodegradable.

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of read across substance Sulfanilic acid (J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 100 mg/l and initial test substance conc. used in the study was 30 mg/l. The percentage degradation of test substance was found to be 3% by BOD, 4% by TOC removal and 1% by HPLC in 28 days. Thus, the substance Sulfanilic acid is not expected to be readily biodegradable in water.

Biodegradation study was conducted according to MITI Test for 14 days for evaluating the percentage biodegradability of read across substance Sulfanilic acid (HSDB, 2016). Conc. of inoculum used for the study was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to be 8% by ThBOD in 14 days Thus, the substance Sulfanilic acid was evaluated to be not readily biodegradable in water.

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of read across substance Sulfanilic acid (GSBL database, 2016). Initial test substance conc. used in the study was 2 mg/l. The percentage degradation of test substance was found to be 55% by using BOD and COD parameter in 28 days. Thus, the substance Sulfanilic acid is not expected to be readily biodegradable in water.

Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of read across substance 2-Amino-5-chloro-4 -methylbenzene sulfonic acid (J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was found to be 0% by O2 consumption & BOD, 2% by TOC removal and < 0% by Test mat. analysis & UV-Vis in 14 days. Thus, the substance 2-Amino-5-chloro-4-methylbenzenesulfonic acid is not expected to be readily biodegradable in water.

Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of read across substance 2-Amino-5-methylbenzenesulfonic acid(J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was found to be 0% by O2 consumption & BOD and < 0 % by TOC removal, Test mat. analysis & UV-Vis in 14 days. Thus, the substance 2-Amino-5-methylbenzenesulfonic acid is not expected to be readily biodegradable in water.

On the basis of above results for target and read across substances, it can be concluded that the test substance compound 2,4 -Diaminobenzenesulfonic acid can be expected to be not readily biodegradable in nature.