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EC number: -
CAS number: -
ready biodegradability of the test item Reaction mass of
C18 (unsaturated) esters with glycerine oligomers
was determined with a non-adapted activated sludge over a test period
of 28 days in the Modified Sturm Test. The study was conducted
according to OECD 301 B . The
was tested at a concentration of 18 mg/L
with 2 replicates corresponding to a carbon content (TOC) of 11.8 mg C/L
in the test vessels.The test
vessels were incubated at low light conditions and at a temperature of
19.0 – 24.5 °C.
biodegradation of the test item was followed by titrimetric analysis
of the quantity of CO2produced by the respiration of
bacteria. The degradation was stopped on day 28 by acidification of
the test solutions. The last titration was made on day 29 after
residual CO2had been purged from the test solutions over a
period of 24 hours. The percentage CO2production was
calculated in relation to the theoretical CO2production
(ThCO2) of the test item. The biodegradation was calculated
for each titration time.
check the activity of the test system sodium benzoate was used as functional
control. The percentage degradation of the functional
control reached the pass level of 60 % within 7 days and a
biodegradation of 83 % after 28 days.
the toxicity control containing both test and reference item a
biodegradation of 80 % was determined within 14 days and it came to 94
% after 28 days. The biodegradation of the reference item was not
inhibited by the test itemin
the toxicity control.
biodegradation of the test item is shown graphically in
comparison to the readily degradable functional control and the
toxicity control. Both test item replicates reached the 10 % level
(beginning of biodegradation) within 3 days. The course of the
degradation was rapid and the test item replicates reached the pass
level of 60 % within 8 days and a biodegradation of 100 % after 28
test item is classified as
the 10 -day-window and within 28 days.
of the Test Item Reaction
mass of C18 (unsaturated) esters with glycerine oligomers in
Comparison to the Functional Control and Toxicity Control
Study Day [d]
Test Item, 1stReplicate
Test Item, 2ndReplicate
Toxicity Controltest item + reference item
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