Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test follows accepted Guidelines available in 1988, no GLP, limited but sufficient reporting

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
50 mg of test substance were weighted in a 400ml flask, diluted with water and homogenised by ultra turrax and ultrasound. The solution was transferred to the test container under continouous stirring and another 2h stirred with a KPG-stirrer.

Solution was turbid and deposit occured at water surface.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
l=2.5-3.4 cm
x=2.9 cm
s=0.27
n=10/concentration

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
none

Test conditions

Hardness:
not reported
Test temperature:
Temp:21-23°C
pH:
pH(Control): 7.6-8.0
pH(limit):7.3-8.0
Dissolved oxygen:
O2(Control): 7.4-9.3 mg/l
O2(limit): 5.4-9.4 mg/l
Salinity:
not reported
Nominal and measured concentrations:
Nominal concentration: 0, 5000mg/l
Details on test conditions:
The test substance was weighted in a 400ml flask, diluted with water and homogenized by ultra turrax and ultrasound. The solution was transferred to the test container under continuous stirring resulting in a 5 g/l concentration and was stirred for two additional hours by a KPG stirrer.
The solution was turbid and deposit occurred at water surface.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
In a range finding study 2 zebrafish have been exposed in concentrations 100, 500 , 1000 and 5000 mg/l. No mortalities have been observed.
In the limit test 10 zebrafish have been exposed to 0 mg/l (control) and 5000mg/l nominal loading. No morthality and no other symptoms have been observed.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The acute fish toxicity of the registration substance was tested in a limit test following OECD203 resulting in a NOEC (96h) of 5000 mg/L nominal. No mortality and no other symptoms have been observed. It is important to note that the measured water solubility of the test substance is 25 mg/L at 20°C.