Reaction products resulting from the esterification of C18 unsaturated fatty acid with glycerol and glycerol oligomers

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test follows accepted Guidelines available in 1988, no GLP, limited but sufficient reporting

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

50 mg of test substance were weighted in a 400ml flask, diluted with water and homogenised by ultra turrax and ultrasound. The solution was transferred to the test container under continouous stirring and another 2h stirred with a KPG-stirrer.

Solution was turbid and deposit occured at water surface.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

l=2.5-3.4 cm
x=2.9 cm
s=0.27
n=10/concentration

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none

Hardness:

not reported

Test temperature:

Temp:21-23°C

pH:

pH(Control): 7.6-8.0
pH(limit):7.3-8.0

Dissolved oxygen:

O2(Control): 7.4-9.3 mg/l
O2(limit): 5.4-9.4 mg/l

Salinity:

not reported

Nominal and measured concentrations:

Nominal concentration: 0, 5000mg/l

Details on test conditions:

The test substance was weighted in a 400ml flask, diluted with water and homogenized by ultra turrax and ultrasound. The solution was transferred to the test container under continuous stirring resulting in a 5 g/l concentration and was stirred for two additional hours by a KPG stirrer.
The solution was turbid and deposit occurred at water surface.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

In a range finding study 2 zebrafish have been exposed in concentrations 100, 500 , 1000 and 5000 mg/l. No mortalities have been observed.
In the limit test 10 zebrafish have been exposed to 0 mg/l (control) and 5000mg/l nominal loading. No morthality and no other symptoms have been observed.

Validity criteria fulfilled:

yes

Conclusions:

The acute fish toxicity of the registration substance was tested in a limit test following OECD203 resulting in a NOEC (96h) of 5000 mg/L nominal. No mortality and no other symptoms have been observed. It is important to note that the measured water solubility of the test substance is 25 mg/L at 20°C.

Description of key information

The acute fish toxicity of the registration substance was tested in a limit test following OECD203 resulting in a NOEC (96h) of 5000 mg/L nominal. No mortality and no other symptoms have been observed. It is important to note that the measured water solubility of the test substance is 25 mg/L at 20°C.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

EC50

Effect concentration:

> 5 000 mg/L