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Ecotoxicological information

Toxicity to soil microorganisms

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Administrative data

Endpoint:
toxicity to soil microorganisms
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No guideline study, but well performed and well documented.

Data source

Reference
Reference Type:
publication
Title:
Effect of metals and other inorganic-ions on soil microbial activity - soil dehydrogenase assay as a simple toxicity test.
Author:
Rogers J.E., Li S.W.
Year:
1985
Bibliographic source:
Bulletin of Environmental Contamination and Toxicology 34/6: 858-865.

Materials and methods

Principles of method if other than guideline:
Dehydrogenease process.
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
7487-88-9
Cas Number:
7487-88-9
IUPAC Name:
7487-88-9
Constituent 2
Reference substance name:
Magnesium sulphate
EC Number:
231-298-2
EC Name:
Magnesium sulphate
IUPAC Name:
231-298-2
Constituent 3
Reference substance name:
magnesium sulphate
IUPAC Name:
magnesium sulphate
Details on test material:
reagent grade Magnesium sulphate.

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no

Test organisms

Test organisms (inoculum):
soil

Study design

Total exposure duration:
24 h

Test conditions

Test temperature:
27°C
Moisture:
22% of water holding capacity (400 g air dry soil + 87 ml distilled water)
Details on test conditions:
pH 7.6
2 replicates per treatment
incubation in the dark
1 g air-dry soil was amended with 0.2 mL of a 3% (w/v) solution of 2, 3, 4-triphenyl tetrazolium chloride (TTC) and 0.5 ml of a 0.5% (w/v) glucose solution in 25 mL centrifuge tubes
soil dehydrogenase activity was assessed as the mg TTC-formazan produced per kg soil per 24 h.
Nominal and measured concentrations:
nominal: 0, 30, 150, 300, 500, 1000, 3000, 5000 mg Mg/kg dry weight
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
> 5 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
magnesium
Basis for effect:
other: dehydrogenase
Remarks on result:
other: Natural surface soil enriched with 1% alfalfa; 3% inhibition at highest dose tested.
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
> 5 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
magnesium
Basis for effect:
other: dehydrogenase
Remarks on result:
other: Natural surface soil without alfalfa; 0% inhibition at highest dose tested.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No guideline study, but well performed and well documented. However, only unbounded NOEC/EC10 values were reported and therefore these values were not considered for derivation of the PNEC, but used as supporting information.

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