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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20 July 1987 to 1 August 1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
50 mL aliquots of test aquaria water were transferred to 70 mL culture tubes. 10 mL of toluene was added to each sample and samples were shaken for three minutes. The toluene phase was then transferred to clean culture tubes and further diluted with toluene when necessary prior to analysis.
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: in house culture
- Age at study initiation (mean and range, SD): < 24 hours old
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
238-278 mg/L as CaCO3
Test temperature:
20 ºC
pH:
8.2-8.3
Dissolved oxygen:
7.7-8.3 mg/L
Nominal and measured concentrations:
0.74, 1.2, 2.7, 4.8 and 10 mg/L (nominal)
0.62, 1.0, 2.0, 3.8 and 8.9 mg/L (measured)
Details on test conditions:
TEST SYSTEM
- Test vessel: glass beakers
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: volume - one litre; covering - 50 mesh stainless steel screen
- Type of flow-through: proportional diluter
- Renewal rate of test solution (frequency/flow rate): 6.2 times per 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water
- Total organic carbon: 0.6-3.8 mg/L
- Particulate matter: 0-1.3 mg/L
- Metals: <0.1 mg/L
- Chlorine: <0.05 mg/L
- Alkalinity: 300-320 mg/L (as CaCO3)
- Conductivity: 480-540 µmhos/cm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): one every 24 hours for mortality and abnormal effects

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.74, 1.2, 2.7, 4.8 and 10 mg/L
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
6.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Remarks on result:
other: 95 % C.I. 3.8-8.9 mg/L
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Details on results:
Mortality rates and observed abnormal behaviour are presented in Table 1.

Table 1: Percentage mortality and percentage abnormal effects

     % mortality  % abnormal effects
 Nominal concentration (mg/L)  Mean measured concentration (mg/L)  24 h  48 h  24 h  48 h
 Control  -  0  0  100 N 100 N
 Solvent control  -  0  0  100 N  100 N
 0.74  0.62  0  0  100 N  100 N
 1.2  1.0  0  0  100 N  100 N
 2.7  2.0  0  0  100 N  88 OB, 8 ERR, 5 N
 4.8  3.8  0  0  90 N, 10 OB  92 OB, 8 ERR
 10  8.9  15  95  65 OB/Q, 18 N. 2SUR  5 OB

N = normal

S = surfacing

ERR = erratic movement

OB = on bottom

Q = quiescent

Validity criteria fulfilled:
not specified
Conclusions:
Under the conditions of the test, the 48 hour LC50 was 6.2 mg/L (95 % C.I. 3.8-8.9 mg/L). The NOEC was determined to be 1.0 mg/L.
Executive summary:

In a GLP compliant acute Daphnia toxicity study conducted in line with standardised guideline EPA OPP 72-2, the acute toxicity to Daphnia of the test material was determined. Daphnia were exposed to the test material at nominal test concentrations of 0, 0.74, 1.2, 2.7, 4.8 and 10 mg/L (equivalent to mean measured concentrations of 0.62, 1.0, 2.0, 3.8 and 8.9 mg/L). Under the conditions of the test, the 48 hour LC50of the test material was determined to be 6.2 mg/L. The 48 hour NOEC was determined to be 1.0 mg/L.

Description of key information

The 48 hour LC50 of the test material was determined to be 6.2 mg/L and the NOEC was determined to be 1.0 mg/L according to a study performed in line with EPA Guideline OPP 72-2.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
6.2 mg/L

Additional information

In the key GLP compliant acute Daphnia toxicity study conducted in line with standardised guideline EPA OPP 72-2, the acute toxicity to Daphnia of the test material was determined. Daphnia were exposed to the test material at nominal test concentrations of 0, 0.74, 1.2, 2.7, 4.8 and 10 mg/L (equivalent to mean measured concentrations of 0.62, 1.0, 2.0, 3.8 and 8.9 mg/L). Under the conditions of the test, the 48 hour LC50 of the test material was determined to be 6.2 mg/L. The 48 hour NOEC was determined to be 1.0 mg/L.

In a supporting GLP compliant acute Daphnia toxicity study conducted in line with standardised guideline EPA OPP 72-2, the acute toxicity to Daphnia of the test material was determined. Under the conditions of the test, the 48 hour EC50 was determined to be 16 mg/L; the 48 hour NOEC was determined to be 2.4 mg/L.

In a supporting GLP compliant acute toxicity study to the mysid conducted in line with standardised guideline EPA OPP 72-3, the acute toxicity to the mysid of the test material was determined. Under the conditions of the test, the 96 hour LC50 was determined to be 2.37 mg/L; the 96 hour NOEC was determined to be 1.32 mg/L.

In a supporting GLP compliant acute toxicity study to the Eastern Oyster conducted in line with standardised guideline EPA OPP 72-3, the acute toxicity to the Eastern oyster of the test material was determined. Under the conditions of the test, the 96 hour EC50 for shell growth was determined to be 1.92 mg/L; the NOEC could not be determined as all tested concentrations resulted in a significant reduction in shell growth.