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EC number: 214-787-5 | CAS number: 1194-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 December 2002 to 22 May 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: samples were collected from each new batch of test solution on days 0 and 1 of the test to determine test material concentrations. Samples were also collected from the 24 hour old solution in each replicate test chamber on day 1 of the test prior to renewal and at test termination. Additional samples were collected on day 2 from the newly prepared batches of test solution prior to renewal and from the 24 hour old solutions in each replicate test chamber and stored for later analysis if required.
- Sampling method: decanting solution from mixing vessels or pipetting of solutions from mid-depth in each test chamber.
- Sample storage conditions before analysis: none - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: test solutions prepared daily at nominal concentrations by added calculated amount of test material into dilution water and mixing for 22-24 hours
- Evidence of undissolved material (e.g. precipitate, surface film, etc): the test material appeared on the surface at test initiation in 12 mg/L solution - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Feeding during test: none (and two days prior to the start of the test)
ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test or not): yes
- Feeding frequency: daily - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 131 mg/L as CaCO3
- Test temperature:
- 11.4 - 13.0 °C
- pH:
- 8.1 - 8.5
- Dissolved oxygen:
- 5.8 - 9.9 mg/L
- Nominal and measured concentrations:
- 0.38, 0.75, 1.5, 3.0, 6.0 and 12 mg/L (nominal)
0.31, 0.62, 1.3, 2.6, 5.5 and 9.4 mg/L (actual) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 4 L glass jars with Teflon-lined lids
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: minimal headspace
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well on the Wildlife International site
- Metals: Mg 11.6 ppm; K 5.45 ppm; all others <5.1 ppm
- Pesticides: <250 ppb
- Alkalinity: 180 mg/L as CaCO3
- Conductivity: 310 µmhos/cm
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/8 hours dark
- Light intensity: 149 lux - Reference substance (positive control):
- no
- Key result
- Duration:
- 96
- Dose descriptor:
- LC50
- Effect conc.:
- 7.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95 % C.I. 5.5-9.4 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Details on results:
- Daily observations of mortality and other signs of toxicity are detailed in Table 1.
All fish in the negative control group appeared healthy and normal throughout the test. All fish in the 0.31, 0.62, 1.3 and 2.6 mg/L treatment groups also appeared normal throughout the test with no mortalities or overt signs of toxicity observed.
While no mortalities occurred among fish in the 5.5 mg/L treatment, sublethal effects were noted throughout the test. - Sublethal observations / clinical signs:
Table 1: Cumulative mortality and clinical observations
3.5 h 24 h 48 h 72 h 96 h Measured conc. (mg/L) Dead Effects Dead Effects Dead Effects Dead Effects Dead Effects % cumulative mortality 0 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0 0 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0 0.31 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0 0.31 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0 0.62 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0 0.62 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0 1.3 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0 1.3 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0 2.6 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0 2.6 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0/10 10AN 0 5.5 0/10 7R; 1E; 1N; 1C 0/10 10R 0/10 10R 0/10 9R; 1N 0/10 9R; 1N 0 5.5 0/10 6R; 3E; 1N 0/10 8R; 1A; 1E 0/10 9R; 1A 0/10 7R; 3A 0/10 9R; 1N 0 9.4 0/10 10R 10/10 - 10/10 - 10/10 - 10/10 - 100 9.4 0/10 10R 10/10 - 10/10 - 10/10 - 10/10 - 100 AN = appear normal
R = lying on the bottom with little motion other than gill movement
E = erratic swimming
N = loss of equilibrium
C = lethargy
A = surfacing for an unusually long period
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of the test, the 96 hour LC50 was 7.2 mg/L (95 % C.I. 5.5-9.4 mg/L). The 96 hour no-mortality concentration was 5.5 mg/L and the NOEC was 2.6 mg/L.
- Executive summary:
In a GLP compliant acute fish toxicity study conducted in line with standardised guidelines OECD 203, EU Method C.1 and EPA OPPTS 850.1075, the acute toxicity to fish of the test material was determined.
Rainbow trout were exposed to the test material at nominal test concentrations of 0.38, 0.75, 1.5, 3.0, 6.0 and 12 mg/L (equivalent to measured test concentrations of 0.31, 0.62, 1.3, 2.6, 5.5 and 9.4 mg/L respectively).
Under the conditions of the test, the 96 hour LC50 of the test material was determined to be 7.2 mg/L. The 96 hour no-mortality concentration was 5.5 mg/L and the NOEC was 2.6 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 09 May 1995 to 21 December 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: EPA Guideline No. 72-3(a)
- Deviations:
- yes
- Remarks:
- (The test temperature slightly exceeded the range of 22 ± 1ºC, the dissolved oxygen concentration fell below 60%. These deviations did not affect the outcome or the validity of the study)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Prior to test initiation, water samples were collected from all test chambers to verify proper diluter function. 4 mL water samples were collected from the controls and all five test solutions at test initiation (day 0) and termination (day 4) to verify actual test concentrations. Water samples were collected from midway in the water column in the test chambers
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 40.4858 and 4.0491 g of test material were weighed into 500 and 50 mL volumetric flasks respectively and brining to volume with dimethylformamide. 784 µL if stock solution was injected in the chemical mixing box (total volume 3137 mL) during each diluter cycle providing the highest nominal test concentration of 20 mg/L. The test solution was proportionally diluted in the system to provide the four lower test concentrations.
- Controls: dilution water control and solvent control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): dimethylformamide - Test organisms (species):
- Cyprinodon variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: sheepshead minnow
- Length at study initiation (length definition, mean, range and SD): 3.2-4.5 cm (mean 3.8 ± 0.4 cm)
- Weight at study initiation (mean and range, SD): 0.82-2.28 g (mean 1.54 ± 0.42 g)
- Feeding during test: no
ACCLIMATION
- Acclimation period: 8 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food: frozen brine shrimp and flake food
- Health during acclimation (any mortality observed): no diseases observed or treated - Test type:
- flow-through
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 21.9 - 23.7 ºC
- pH:
- 8.0-8.2
- Dissolved oxygen:
- 4.7-7.5 mg/L (initial)
3.1-5.7 mg/L (during test) - Salinity:
- 20 %
- Nominal and measured concentrations:
- 2.59, 4.32, 7.20, 12 and 20 mg/L (nominal)
2.07, 3.53, 6.44, 10.4 and 16.6 mg/L (actual) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 23.6 L glass tanks
- Material, size, headspace, fill volume: fill volume - 15.3 L and water height of 13 cm; test solution volume - 1400 ± 140 mL
- Type of flow-through: modified proportional vacuum-siphon diluter system
- Renewal rate of test solution (frequency/flow rate): average rate of 4.6 cycles/hour providing 10.1 volume additions every 24 hours
- No. of organisms per vessel: 20
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural saltwater pumped from a shallow saltwater well
- Metals: magnesium 742 mg/L; all others <0.01 mg/L
- Pesticides: <1.2 µg/L
- Chloride: 10,800 mg/L
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/8 hours dark
- Light intensity: 4.9-6.7 microEinsteins per square meter per second
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality (daily); abnormalities in behaviour or physical appearance (daily)
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.01, 0.1, 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes (0 % mortality at ≤10 mg/L; 100 % mortality at 100 mg/L) - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 12.7 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95 % C.I. 10.4-16.6 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.53 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Daily observations of mortality and other signs of toxicity can be found in Table 1.
- Sublethal observations / clinical signs:
Table 1: Cumulative mortality and clinical observations
24 hours 48 hours 72 hours 96 hours Mean measured conc. (mg/L) Dead Effects Dead Effects Dead Effects Dead Effects Cumulative % mortality 0 0/20 20AN 0/20 20AN 0/20 20AN 0/20 20AN 0 0 (solvent control) 0/20 20AN 0/20 20AN 0/20 20AN 0/20 20AN 0 2.07 0/20 20AN 0/20 20AN 0/20 20AN 0/20 20AN 0 3.53 0/20 20AN 0/20 20AN 0/20 20AN 0/20 20AN 0 6.44 0/20 9AN; 11A 0/20 3AN; 17A 0/20 19AN; 1A 0/20 19AN; 1A 0 10.4 1.20 1AN; 18A 1/20 17A;8N 1/20 19N; 19C 1/20 19N; 19C 5 16.6 14/20 6N 18/20 2N 202/20 - 20/20 - 100 AN = appear normal
N = loss of equilibrium
C = lethargy
A = at the surface
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of the test, the 96 hour LC50 was 12.7 mg/L (95 % C.I. 10.4-16.6 mg/L). The 96 hour NOEC was 3.53 mg/L.
- Executive summary:
In a GLP compliant acute fish toxicity study conducted in line with standardised guidelines OECD 203, EU Method C.1 and EPA Guideline No. 1 72 -3(a), the acute toxicity to fish of the test material was determined. Sheepshead minnow were exposed to the test material at nominal test concentrations of 2.59, 4.32, 7.20, 12 and 20 mg/L (equivalent to measured test concentrations of 2.07, 3.53, 6.44, 10.4 and 16.6 mg/L respectively).
Under the conditions of the study, the 96 hour LC50 of the test material was determined to be 12.7 mg/L. The 96 hour NOEC was 3.53 mg/L.
Referenceopen allclose all
Description of key information
The LC50 of the test material, to Rainbow trout, was determined to be 7.2 mg/L and the NOEC to be 2.6 mg/L according to a study performed in line with OECD Guideline 203, EU Method C.1 and EPA OPP 850.1075.
The LC50 of the test material, to Sheephead minnow, was determined to be 12.7 mg/L and the NOEC to be 3.53 mg/L according to a study performed in line with OECD 203, EU Method C.1 and EPA Guideline No. 1 72 -3(a).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 7.2 mg/L
Additional information
Oncorhynchus mykiss and Cyprinidon variegatus were similar in sensitivity to the test material with 96 hour LC50 values of 7.2 mg/L and 12.7 mg/L, respectively. In addition, to these two GLP studies, a review has been reported on the acute toxicity of the test material. For the test material, the 96 hour LC50 values (excluding ‘greater than values' which appear to represent outliers) ranged from 4.2 to 42 mg/L. Based on the review by Gijswijt (1980), three out of 56 endpoints, representing 24 species of fish, were lower but very similar to the endpoint for O. mykiss in the GLP study; these were 6.5, 4.2 and 6.4 mg/L. As the results from the C. variegatus study were obtained from only one replicate, the O. mykiss study is considered to be the key study for this endpoint.
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