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EC number: 229-355-1
CAS number: 6486-23-3
Mortality of Adult Earthworms in
[%] after 28 Days of Exposure
[mg test item/kg soil dry weight]
Earthworm Behaviour and
Pathological Symptoms after 28 Days of Exposure
A) No observation of symptoms or behavioural changes; H) Dead earthworms
Body Weight Changes of the Adult
[mg test item /kg soil dry weight]
Mean body weights per replicate
Mean body weight change of earthworms
Mean ± SD
Sig. to pooled control
0.13 ± 0.04
26.8 ± 8.15
0.12 ± 0.03
25.2 ± 5.46
0.13 ± 0.03
26.0 ± 0.03
0.12 ± 0.05
24.0 ± 10.1
Reproduction Rate (Number of
Juveniles after 8 Weeks)
Number of juveniles
of pooled control
Significance compared to pooled control
146 ± 5.89
135 ± 21.6
140 ± 16.4
134 ± 17.8
of the test item on mortality, biomass and the reproductive potential of
the earthworm species Eisenia fetida (Savigny) were determined
according to the Guideline OECD 222 (2004). The study was conducted
under static conditions over 8 weeks with a limit test item
concentration of 1000 mg test item/kg soil dry weight, that was mixed
into artificial soil containing 10 % peat. A control using untreated
artificial soil and a solvent control with the solvent mixed into the
artificial soil were set up. 80 test organisms were inserted into 8
control, solvent control and test item replicates (10 earthworms per
replicate). They had an individual body weight between 0.30 and 0.60 g
at experimental starting.
days of exposure in soil, no evident earthworm mortalities as well as no
pathological symptoms or changes in the behaviour of adult earthworms
were observed in all treatments. After 28 days of exposure, no
statistically significant differences in earthworm body weights at 1000
mg/kg soil dry weight were observed. After further four weeks the
reproduction rate (average number of juveniles produced) was 140 in the
pooled control and ranged between 104 and 158 in the test item treatment
rate (arithmetic mean: 134). Compared to the pooled control,
reproduction rate was not statistically significantly reduced at 1000
mg/kg soil dry weight. As
there were no statistically significant differences determined between
the control and the solvent control for the body weight and the
reproduction rate, the controls were pooled.
the NOEC of the test item concerning mortality, biomass and reproduction
of earthworms was 1000 mg/kg soil dry weight.
validity criteria recommended by the test guideline were fulfilled.
Summary of All Observed Effects in
the Tested Application Rates
Mortality of adult earthworms
Reduction of body weight
Reduction of reproduction rate
(Number of juveniles)
Summary of all effects
NOEC and LOEC values
NOECreproduction, mortality, body weight
LOECreproduction, mortality, body weight
The study used as source investigated C.I. Pigment Yellow 1. The study results of the source compound were considered applicable to the target compound. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13 or find a link in cross reference “assessment report”.
Under the conditions of this study, the test item did not induce
mortality, pathological symptoms or changes in the behaviour of adult
earthworms after 28 days of exposure at the limit test item
concentration. The adult earthworm biomass increased in the control and
at the limit test item concentration without any statistically
significant differences. After eight weeks of exposure the earthworm
reproduction in the limit test item concentration did not statistically
significantly differ to the control.
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