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EC number: 229-355-1
CAS number: 6486-23-3
Data and Respiration Rates of Test and Reference Item Concentrations
determined at the start of the 3 minute measurement
5 mm are related to 0.1 mgO2/L
Rates and Inhibition of Respiration of Test and Reference ItemConcentrations
of the control respiration rates: 12 %
A Respiration Inhibition Test with activated sludge according to OECD
Guideline No. 209 and compliant to GLP (reliability category 1) was
performed for 3 h with the submission substance. Test system was
activated sludge of the domestic treatment plant of D-31157 Sarstedt.
The test was performed under static conditions with the nominal
concentration 1000 mg/L. The respiration rates of control, reference and
test item concentrations were measured after a contact time of three
hours, and the inhibitory effects of the test and reference
concentrations were determined in comparison to the control respiration
rates. No inhibition was observed. In order to check the activity of the
test system and the test conditions a reference test was carried out
with copper-(II)-sulphate-pentahydrate as reference item and the
reference toxicity was determined. The EC50- value for the reference
item was 125 mg/L. Validity criteria of the test guideline were
fulfilled. The submission substance is not toxic at concentrations up to
1000 mg/L to activated sludge of a domestic sewage treatment plant.
In a respiration inhibition test with activated sludge according to OECD
209 no respiration inhibition was observed at the nominal test item
concentration of 1000 mg/L (limit test).
EC50 (3h) > 1000 mg/L (nominal)
NOEC (3h) = 1000 mg/L (nominal)
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