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EC number: 229-355-1
CAS number: 6486-23-3
of Juveniles in the Control and Test Group after 21 d
Mean No. of Juveniles
Number of Juveniles in Replicate No.
MV ± SD
126 ± 4
126 ± 11
[%] of the Adult Daphnids after 7, 14 and 21 d of Exposure
Test Item Concentration[mg/L]
Number of Dead (Stillborn + Aborted Eggs) to Total Number of Juveniles
NominalTest Item Concentration[mg/L]
Number of Juveniles
Percentage ofDead Juveniles*[%]
Dead = Aborted eggs + stillborn juveniles
Total = Deasd + alive juveniles
* = Related to the total number of juveniles
Appearance of Living Juveniles in the Individual Groups
Day of First Appearance of Living Juveniles
in Replicate No.
Length and Dry Weight of the Parental Daphnids
Total Length of the Parental Daphnids [mm]
Dry Weight [mg]
N= number of parental daphnids alive
The Daphnia magna Reproduction
Test (Semi-Static with daily renewal, 21 d) ot the test item was
conducted as a limit test with the saturated solution with 1.00 mg/L
test item according to OECD 211 (2008).
The selected test organism was Daphnia magna STRAUS (Clone 5).
Ten daphnids individually held were used for the saturated test item
solution and the control, respectively. At test start the daphnids were
2 to 24 h old. The test method was semi-static with a daily renewal of
the test solutions. The aim of the Daphnia Reproduction Test over 21
days was to assess effects on the reproduction capacity and other test
item-related effects, including occurrence of aborted eggs and stillborn
juveniles, time of production of first brood, adult mortality, intrinsic
rate of natural increase, dry body weight and length of the parent
The tested saturated solution of the test item and the control were
analytically verified by HPLC-DAD of samples on days 0, 7, 14 (fresh
media, 0 hours) and on days 1, 8, 15 (old media, 24 hours).
Due to the very low solubility of the
test item it was not possible to measure the test item concentrations in
samples of the fresh (0 hours) and the old media (24 hours) of the
saturated solution and the control. The results given are based on the
nominal concentration of 1.00 mg/L of the test item.
average values of juveniles per parental daphnid were 126
for the control and also for the saturated test item solution with 1.00
mg test item/L after 21 days. The reproductive output of daphnids in the
saturated test item solution* (limit concentration) was comparable to
the output of daphnids in the control (Mann-Whitney
Rank Sum Test,
coefficient of variation of the number of living offspring produced
per parent was 9 % for the control and 4 % for the saturated
test item solution, respectively.
intrinsic rates of natural increase (IR) of the surviving parent
daphnids accounting for generation time and number of offspring were
calculated as a measure for population growth and maintenance. The IR of
the saturated solution was
the control group (t-test,
p = 0.05).
or immobilization of parental daphnids was observed
in the control and the
to the total number of produced juveniles (dead + alive) the percentage
of dead juveniles and aborted eggs was lower than 1 % in the
saturated solution, which is seen to be not biologically significant. In
the control no dead juveniles or aborted eggs were observed.
broods, respectively, were observed during the test period for all
parental daphnids. The first brood was released from all parent
daphnids in all treatments by day 9.
mean dry body weight and total
body lengths of
all parental daphnids of the test and the control group were determined
at the end of the study.
The mean dry body weight of the parental daphnids was 0.93 mg per
daphnid for the control and 1.10 mg per daphnid for the saturated test
The mean body length of the parental daphnids was 5.50 mm
per daphnid for the control and 5.40 mm per daphnid for the saturated
test item solution.
ephippia (winter eggs) were
observed in the respective control or test groups.
of all test item related effects is given in Table 1.
No biologically or statistically significant effects could be determined
in the saturated test item solution with 1.00 mg/L test item when
compared to the control.
Summary of all
Test Item Related Effects
on the nominal concentration of the test item of
Mean Number of Juvenilesper Producing Parent(Reproduction Rate ± SD)
Coefficient of Variation of the Mean Number of Juveniles per Producing Parent
Intrinsic Rate ofNatural Increase (Mean ± SD)
0.47 ± 0.02
0.47 ± 0.02
Appearance of First Brood (Mean Day ± SD)
8.4 ± 0.5
8.7 ± 0.5
Number of Broods
Stillborn Juveniles and Aborted Eggs
Adult Mortality after 21 Days [%]
Parent Daphnids:Mean Dry Weight [mg]
Parent Daphnids:Mean Body Length [mm] ± SD
5.50 ± 0.17
5.40 ± 0.24
quality parameters (pH-value, dissolved oxygen, water hardness and
temperature) were determined to be within the acceptable limits.
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