Registration Dossier

Environmental fate & pathways

Additional information on environmental fate and behaviour

Currently viewing:

Some information in this page has been claimed confidential.

Administrative data

Endpoint:
additional information on environmental fate and behaviour
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Remarks:
Non-GLP, non-guideline study following sound scientific principles. This is a non-standard, proprietary study specifically designed and conducted in order to provide supporting evidence to the testing program. Acceptable with restrictions.

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Ecotoxicity screening studies were conducted in which a biomimetic extraction technique using solid-phase microextraction (SPME) in conjunction with gas chromatography (GC) was employed to assess the level of water soluble hydrocarbons and hence the potential toxicity of the water accommodated fractions (WAFs) of the test samples. In addition, the toxicity of WAFs was also monitored using an in vitro Microtox assay, a biosensor-based measurement system.
GLP compliance:
no
Type of study / information:
Leaching of the thickener from base oil to water for a range of greases

Results and discussion

Any other information on results incl. tables

Results and Conclusions

The SPME and Microtox results for the WAFs of the grease samples at 1000 mg/L loading rate are as follows:

Grease sample SPME-GC Microtox reading
(Total peak area) (5 min) (15 min)

C - Polyurea thickener 2 in base oil

503,192

>100

>100

Both sets of screening data confirm that there is no apparent toxicity associated with any of the samples at the 1000 mg/L loading rate. The SPME-GC data summarised above, supported by the chromatograms, confirm that the total peak area counts are all extremely low and equivalent to background readings associated with a control sample (i.e. a WAF without any test sample added).

The screening ecotoxicity data (using SPME-GC and the in vitro Microtox assay), confirm a lack of toxicity of the greases.

Applicant's summary and conclusion

Conclusions:
There is negligible leaching of polyurea thickener 2 from base oil in the samples tested. The SPME-GC data confirm that the total peak area counts are all extremely low and equivalent to background readings associated with a control sample.
Executive summary:

Leaching studies have been undertaken on thickeners in base oils to provide additional support to the grease thickener testing strategy. Information generated during the screening studies is of relevance and value to the risk assessment of a wide range of grease thickeners and for assessing the potential bioaccessibility of grease and other lubricant components. The approach for the polyurea thickeners was to use a solid-phase microextraction (SPME) method combined with gas chromatography (GC) to assess the bioaccessibility of polyurea thickeners in water accommodated fractions (WAFs) (i.e. the leaching of the thickener from base grease into water). Ecotoxicity screening studies were conducted in order to determine whether there was any toxicity associated with the grease samples. The toxicity of WAFs was monitored using an in vitro Microtox assay, a biosensor-based measurement system. The screening ecotoxicity data (using SPME-GC and the in vitro Microtox assay), confirm a lack of toxicity of the greases.