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Ecotoxicological information

Toxicity to terrestrial arthropods

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Reference
Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
according to
Guideline:
other: Data requirements for supporting registration of pesticides (Notification No.12-Nousan-8147)
Deviations:
no
GLP compliance:
no
Application method:
spray
Vehicle:
yes
Remarks:
Distilled water (with 0.01% Tween 20)
Test organisms (species):
other: pardosa pseudoannulata
Animal group:
Araneae (soil-dwelling lycosid spider)
Study type:
laboratory study
Limit test:
yes
Total exposure duration:
72 h
Post exposure observation period:
2, 24, 48 and 72 hours
Test temperature:
25°C
Photoperiod and lighting:
16 hour photoperiod (16 hours light/ 8 hours dark)
Nominal and measured concentrations:
Nominal: 0.15 µg ai/cm² (15 g/ha) (in a volume equivalent to 600 L water/ha)
Reference substance (positive control):
yes
Remarks:
Dimethoate
Key result
Duration:
72 h
Dose descriptor:
LR50
Effect conc.:
> 15 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Validity criteria fulfilled:
yes
Conclusions:
72-Hr LR50 (Wolf Spider): >15 g/ha (mortality) (Highest concentration tested)
Executive summary:

The study was conducted according to guideline Data requirements for supporting registration of pesticides (Notification No. 12-Nousan-8147) to determine acute 72-hour toxicity of test substance in the predatory spider, Pardosa pseudoannulata (wolf spider). The test organisms, second instar larval spiderlings, were exposed for 72 hours to an untreated control and to fresh dried residues of test substance applied to moistened quartz sand at a single nominal rate of 0.15 µg ai/cm², equivalent to a field rate of 15 g/ha in 6 µL of water per cm², a volume equivalent to 600 L water/ha. A wetting agent, Tween 20, was included at a rate of 0.01% v/v in the spray mixture. A toxic reference (dimethoate) was included in the test at 2.58 µg a.i./6 µL/cm² (258 g dimethoate/ha). The test was comprised of three replicates of 10 larvae for the treated group, and 10 larvae each for the control and toxic reference groups. Assessments for mortalities were carried out 2, 24, 48 and 72 hours after treatment.

Under the conditions of the study, the 72-hour LR50 of test substance in Pardosa pseudoannulata based on mortality and nominal rates was >15 g/ha, the highest concentration tested.

 

Description of key information

72-Hr LR50 (Wolf Spider) > 15 g/ha; Notification 12-Nousan-8147; Reliability = 2

Key value for chemical safety assessment

Additional information

72-hr Oral LD50 (Hymenoptera, honeybee) = 0.51 μg/bee; OECD 213,214; Reliability = 1

72-hr Dermal LD50 (Hymenoptera, honeybee) = 0.39 μg/bee; OECD 213,214; Reliability = 1

72-hr Oral NOEC (Hymenoptera, honeybee) = 0.16 μg/bee; OECD 213,214; Reliability = 1

72-hr Dermal NOEC (Hymenoptera, honeybee) = 0.13 μg/bee; OECD 213,214; Reliability = 1

96-hr Oral LD50 (Apis Mellifera, honeybee) = 1 μg/bee; OECD 213,214; Reliability = 2

96-hr Dermal LD50 (Apis Mellifera, honeybee) = 10 μg/bee; OECD 213,214; Reliability = 2

72-hr LR50 (Lepidoptera, parasitic wasp) > 5 g/ha; Notification 12-Nousan-8147; Reliability = 2

7 d mortality rate (Bombyx mori, silkworm) = 93.3%; Notification 12-Nousan-8147; Reliability = 2

96 h LC50 (Bombyx mori, silkworm) = 9.45 mg a.i./L; China Regulation Part 11; Reliability = 2

24-Hr LR50 (Trichogramma) > 0.0025 mg a.s./cm²; China Regulation Part 11; Reliability = 2

72-Hr LR50 (Orius strigicollis) > 5 g/ha; Notification 12-Nousan-8147; Reliability = 2