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Sediment toxicity

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Reference
Endpoint:
sediment toxicity: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 235 (Chironomus sp., Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
other: JMAFF 12-Nousan-8147 (2000), 2-7-6 (2013)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Chironomus riparius
Test type:
static
Water media type:
freshwater
Limit test:
no
Duration:
48 h
Post exposure observation period:
Approximately 5.5, 24 and 48 hours (± 1 hour)
Hardness:
144 mg/L (as CaCO3)
Test temperature:
19.9 to 20.8°C
pH:
8.2 to 8.5
Dissolved oxygen:
≥8.5 mg/L (≥94% of saturation)
Conductivity:
324 μS/cm
Nominal and measured concentrations:
Nominal: 0.48, 1.1, 2.3, 5.1, 11, 25, 55 and 120 mg a.s./L
Measured: 0.46, 1.1, 2.3, 4.9, 11, 24, 54 and 118 mg a.s./L
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: immobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.48 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: immobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: immobility
Validity criteria fulfilled:
yes
Conclusions:
48-Hr EC50 (Midge): 2.5 mg a.s./L (Immobility)
Executive summary:

The study was conducted according to guideline OECD Guideline 235 to determine acute toxicity of test substance to unfed Chironomus riparius larvae (3 days old) in an unaerated, static, 48-hour test.

 

Treatments consisted of a dilution water control and eight nominal concentrations of 0.48, 1.1, 2.3, 5.1, 11, 25, 55 and 120 mg active substance (a.s.)/L and the mean measured concentrations of test substance are 0.46, 1.1, 2.3, 4.9, 11, 24, 54 and 118 mg a.s./L. and ranged from 96 to 100% of nominal concentrations. Five midges were used per replicate with four replicates per test concentration and control and Immobility and behavioral observations were made at approximately 5.5, 24 and 48 hours (± 1 hour) following initiation of exposure. The highest nominal test concentration causing no immobility at test end was <0.48 mg a.s./L, the lowest concentration tested. The lowest nominal test concentration causing 100% immobility at test end was 25 mg a.s./L. The 48-hour EC50, based on nominal concentrations and immobility data, was 2.5 mg a.s./L, with a 95% confidence interval of 1.6 to 3.7 mg a.s./L

Description of key information

48 -hr EC50 (Chironomus riparius, Midge) = 2.5 mg/L; OECD 235; Reliability = 1

Key value for chemical safety assessment

Additional information

In the midge, the 48-hour EC50 was 2.5 mg/L.