Registration Dossier

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
no
Qualifier:
according to
Guideline:
ISO 11268-1 (Effects of Pollutants on Earthworms. 1. Determination of Acute Toxicity Using Artificial Soil Substrate)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
yes
Remarks:
Quartz sand
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
18.0 to 22.0°C
pH:
Test initiation: 7.52 to 7.59
Test termination: 7.20 to 7.27
Moisture:
Initiation: 24.82 to 25.85% (equivalent to 57.49 to 59.88% of the total water holding capacity)
Termination: 24.09 to 26.13% (equivalent to 55.80 to 60.53% of the total water holding capacity)
Nominal and measured concentrations:
0, 62.5, 125, 250, 500 and 1000 mg/kg dry soil
Reference substance (positive control):
yes
Remarks:
2-Chloroacetamide (tested twice per year)
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Highest dose tested
Key result
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
< 62.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: biomass
Key result
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Highest dose tested
Validity criteria fulfilled:
yes
Conclusions:
7 d and 14 d LC50 (Eisenia fetida): >1000 mg/kg dry soil (mortality)
14 d NOEC (Eisenia fetida): <62.5 mg/kg dry soil (biomass)
14 d NOEC (Eisenia fetida): 1000 mg/kg dry soil (mortality)
Executive summary:

The acute toxicity of the test substance to the earthworm, Eisenia fetida (Savigny), was estimated in a 14-day soil exposure laboratory study conducted as per OECD Guideline 207 and ISO-Guideline 11268-1. Four replicates of ten clitellated adult earthworms were each exposed to nominal concentrations of 62.5, 125, 250, 500 and 1000 mg/kg dry soil. The untreated control (Quartz sand) was replicated four times, with ten earthworms in each replicate.

The toxic reference, 2-Chloroacetamide, is tested twice a year. The 14-day LC50 of the most recent test was 30.8 mg Chloroacetamide/kg dry soil.

Earthworms were assessed for mortality and behavioral effects after 7 and 14 days of exposure and earthworm body weights were assessed at day 0 and day 14.

According to the results of this study, the 7-day and the 14-day LC50 of the test substance was determined to be greater than 1000 mg/kg dry soil. The 14-day No-Observed-Effect Concentration (NOEC) with respect to biomass and mortality was determined to be less than 62.5 mg and 1000 mg/kg dry soil, respectively.

Description of key information

14 -day EC50 (earthworm) > 1000 mg/kg; OECD ; Reliability = 1

14-day NOEC ( Eisenia fetida, earthworm) > 1000 mg/kg (mortality); OECD 207; Reliability = 1

14-day NOEC ( Eisenia fetida, earthworm) < 62.5 mg/kg (biomass); OECD 207; Reliability = 1

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
1 000 mg/kg soil dw
Long-term EC10, LC10 or NOEC for soil macroorganisms:
62.5 mg/kg soil dw

Additional information