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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 March 2010 and 16 April 2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
440/2008
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Sponsor's identification: Octafluoropentyl methacrylate
Purity >99%
Analytical monitoring:
yes
Details on sampling:
- Concentrations: control and each test group
- Sampling method: Samples were collected at 0 and 48 hours, replicate test condition samples were pooled
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Diluting (with reconstituted water) a saturated solution prepared from an initial test item dispersion at a concentration of 100 mg/L. The test item was dispersed in reconstituted water with the aid of propeller stirring at approximately 1500 rpm for 24 hours. After 24 hours, the stirring was stopped and any undissolved test item was removed by filtration through a 0.2 µm Sartorius Sartopore filter.
- Controls: reconstituted water
- Evidence of undissolved material: none
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house laboratory cultures
- Age of parental stock: first instar
- Feeding during test: None

ACCLIMATION
- Acclimation period: 24 hours, gravid adults were isolated
- Acclimation conditions: same as test; ensuring parthenogenesis
- Type and amount of food: fed daily with a suspension of algae
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
250 mg CaCO3 / L
Test temperature:
20 - 21 °C
pH:
7.7 - 8.0
Dissolved oxygen:
8.4 to 9.3 mg O2/L
Nominal and measured concentrations:
Nominal Concentration: 1.7, 3.1, 5.4, 9.5, 17 mg/L
Geometric Mean Measured Concentration: 0.96, 1.9, 3.3, 5.8, 11 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 300 mL glass stoppered conical flasks
- Type: closed
- Material, size, headspace, fill volume: containing approximately 300 mL of test preparation; test vessels were completely filled and sealed to minimise losses through volatility
- Aeration: None
- No. of organisms per vessel: 10 organisms/vessel
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Culture medium different from test medium: No
- Intervals of water quality measurement: 0 and 48 hours for pH and dissolved oxygen

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light and 8 hours darkness, with 20-minute dawn and dusk transition periods

EFFECT PARAMETERS MEASURED: immobilisation or adverse reactions recorded 24 and 48 hours after start of exposure

RANGE-FINDING STUDY
- Test concentrations:
Initial range-finding test: 0.017, 0.17, 1.7 and 17 mg/L
Second range-finding test: 0.017, 0.17, 1.7 and 17 mg/L
- Results used to determine the conditions for the definitive study: Based on the results of the range-finding tests and initial experiment the following nominal test concentrations were assigned to the definitive test: 1.7, 3.1, 5.4, 9.5 and 17 mg/I (0.96, 1.9, 3.3, 5.8 and 11 mg/I based on geometric mean measured test concentrations).
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 11 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 100% v/v saturated solution
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 11 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 100% v/v saturated solution
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
11 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 100% v/v saturated solution
Details on results:
- Behavioural abnormalities: None reported
- Other biological observations: None reported
- Mortality of control: 0%
- Other adverse effects control: None
- Abnormal responses: None reported
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Test preparation were observed to be clear, colourless solutions throughout the duration of the test.
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: 48h EC50 0.65 mg/L
Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of the test item to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EC50, based on geometric mean measured test concentrations of greater than 11 mg/L. Correspondingly the No Observed Effect Concentration was 11 mg/L. This study showed that there were no toxic effects at saturation.
Executive summary:

A GLP-complaint study according to OECD Guideline 202 was conducted to assess the acute toxicity of octafluoropentyl methacrylate to Daphnia magna. Following range-finding tests, twenty daphnids (2 replicates of 10 animals) were exposed to dilutions of a 100% v/v saturated solution of test item with geometric mean measured concentration of 0.96, 1.9, 3.3, 5.8 and 11 mg/L for 48 hours at approximately 20 °C under static conditions.

 

No toxic effects at saturation were reported. The 48-hour EC50 based on the geometric mean measured test concentrations was greater than 11 mg/L and correspondingly the No Observed Effect Concentration was 11 mg/L.

Description of key information

A GLP-complaint study according to OECD Guideline 202 was conducted to assess the acute toxicity of octafluoropentyl methacrylate to Daphnia magna. Following range-finding tests, twenty daphnids (2 replicates of 10 animals) were exposed to dilutions of a 100% v/v saturated solution of test item with geometric mean measured concentration of 0.96, 1.9, 3.3, 5.8 and 11 mg/L for 48 hours at approximately 20 °C under static conditions.

 

No toxic effects at saturation were reported. The 48-hour EC50 based on the geometric mean measured test concentrations was greater than 11 mg/L and correspondingly the No Observed Effect Concentration was 11 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
11 mg/L

Additional information