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EC number: 269-950-3
CAS number: 68391-42-4
ErC50 > 100 mg/l
EyC50 > 100 mg/l
No information on toxicity to aquatic
algae and cyanobacteria was available on target substance. Therefore,
available data on Similar Substance 01 was taken into account for the
assessment. More details about the similarity between the two substances
are reported in section 13.
test substance to
unicellular freshwater green alga Pseudokirchneriella subcapitata was
determined according to OECD guideline 201 and EU method C.3.
The study was conducted under static conditions with an
initial cell density of 6839 cells/ml. A saturated solution with a
nominal loading of 100 mg test substance/l was prepared; undissolved
particles were removed by membrane filtration. Thereafter, components of
dilution water were added to saturated solution. Test concentrations
obtained by dilution of saturated solution were: 6.25 - 12.5 - 25.0 -
50.0 - 100 % and were tested in triplicates. As for control, 6
replicates were used.
As test concentrations, measured by
HPLC-DAD, resulted to be below the lowest calibration level (LCL) at
start of exposure, effect values were given as nominal loading levels.
Based on 6 % inhibition of growth rate and 28 % inhibition of yield
after 72 h, ErL50 and EyL50 were both above 100 % (i.e. 100 mg/l).
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