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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from secondary literature.
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: As mentioned below
Principles of method if other than guideline:
Daphnia magna Bioassay was carried out under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate on aquatic environment.
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material: p-Methylaminophenol sulfate- IUPAC name: Bis(4-hydroxy-N-methylanilinium) sulphate- Molecular formula: C14H20N2O6S- Molecular weight: 344.386 g/mole- Smiles:CNc1ccc(cc1)O.CNc1ccc(cc1)O.OS(=O)(=O)O- Inchl: 1S/2C7H9NO.H2O4S/c2*1-8-6-2-4-7(9)5-3-6;1-5(2,3)4/h2*2-5,8-9H,1H3;(H2,1,2,3,4)- Substance type: Organic- Physical state: Solid crystalline (off white - white)
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
- Stock solution: At the start of each bioassay, appropriate dilutions of each test chemical were prepared and dispensed into 150-milliliter Griffin beakers. The pH of each test solution was adjusted to between pH 7.07.5.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM- Common name: water flea- Source: Carolina Biological Supply company, Burlington North carolina.- Method of breeding: A more satisfactory culture medium was obtained by blending approximately 2 grams of alfalfa pellets with 3-4 dried brine shrimp per liter of water. This medium was replaced every 2-3 days and was successful in promoting reproduction of the stock culture.- Food type: algae
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
54.5 (37.0-75.0) F
pH:
7.0-7.5
Details on test conditions:
- Test vessel: one-gallon, wide-mouth, - Material, size, headspace, fill volume: glass jars
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.019 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: : toxic

Bioassay data summary of Daphnia Magna

 

 

 

Cumulative mortalities

Percent

mortality at

 

Test chemical

Conc.(mg/l)

Starting population

24 hr

48

hr

72

hr

96

hr

96 hrs

96hr LC50

or range

(mg/l)

4-methyl

Amino

Phenol

Sulfate

(VIIID8)

0.3

11

2

4

9

9

81.8

0.019

0.09

11

1

3

7

8

72.7

0.03

10

2

3

4

7

70.0

0.009

10

1

1

2

4

40.0

0.0

53

1

2

2

4

7.5

Conclusions:
The lethal concentration LC50 to 50% of daphnia magna is 0.019 mg/L at 96 hrs. Mortality was measured during the test. It can be concluded from the value that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic environment.
Executive summary:

Daphnia magna Bioassay was carried out under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate on aquatic environment. At the start of each bioassay, appropriate dilutions of each test chemical were prepared and dispensed into 150 milliliter Griffin beakers. The pH of each test solution was adjusted to between pH 7.0-7.5. A final volume of 100-110 milliliters was used throughout the testing program. Controls, which contained only Daphnia in dilution water, were included in every bioassay. Adult mortalities were recorded on a daily basis. Births and newborn mortalities observed during the experiment were noted but not enumerated. The lethal concentration LC50 to 50% of daphnia magna is 0.019 mg/L at 96 hrs. It can be concluded from the value that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic environment and can be considered as “aquatic Chronic 1” as per classification for the aquatic environment.

Description of key information

Daphnia magna Bioassay was carried out under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate on aquatic environment. At the start of each bioassay, appropriate dilutions of each test chemical were prepared and dispensed into 150 milliliter Griffin beakers. The pH of each test solution was adjusted to between pH 7.0-7.5. A final volume of 100-110 milliliters was used throughout the testing program. Controls, which contained only Daphnia in dilution water, were included in every bioassay. Adult mortalities were recorded on a daily basis. Births and newborn mortalities observed during the experiment were noted but not enumerated. The lethal concentration LC50 to 50% of daphnia magna is 0.019 mg/L at 96 hrs. It can be concluded from the value that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic environment and can be considered as “aquatic Chronic 1” as per classification for the aquatic environment.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
0.019 mg/L

Additional information

Daphnia magna Bioassay was carried out under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate on aquatic environment. At the start of each bioassay, appropriate dilutions of each test chemical were prepared and dispensed into 150 milliliter Griffin beakers. The pH of each test solution was adjusted to between pH 7.0-7.5. A final volume of 100-110 milliliters was used throughout the testing program. Controls, which contained only Daphnia in dilution water, were included in every bioassay. Adult mortalities were recorded on a daily basis. Births and newborn mortalities observed during the experiment were noted but not enumerated. The lethal concentration LC50 to 50% of daphnia magna is 0.019 mg/L at 96 hrs. It can be concluded from the value that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic environment and can be considered as “aquatic Chronic 1” as per classification for the aquatic environment.