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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from water research journal
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: refer principle below
Principles of method if other than guideline:
Percent biodegradability of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate is determined by COD ( Organic Carbon ) Parameter using adapted activated sludge as inoculums.
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material (as cited in study report): Metol- IUPAC name: Bis(4-hydroxy-N-methylanilinium) sulphate- Molecular formula: C14H20N2O6S- Molecular weight: 344.386 g/moleSmiles:CNc1ccc(cc1)O.CNc1ccc(cc1)O.OS(=O)(=O)O- Inchl: 1S/2C7H9NO.H2O4S/c2*1-8-6-2-4-7(9)5-3-6;1-5(2,3)4/h2*2-5,8-9H,1H3;(H2,1,2,3,4)- Substance type: Organic- Physical state: Solid crystalline (off white - white)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, adapted
Details on inoculum:
- Source of inoculum/activated sludge: from sewage plant- Method of cultivation: Activated sludge is cultivated in 1000 mL volumetric flask and aerated with pressure air for 5 days.(200 ml of the thickened activated sludge) is diluted with tap water to the volume of ca. 800 ml and 600 mg 1-' of starch or glucose 600 mg 1-' of peptone, 25 ml of a phosphate buffer pH 7.2, and the solution of the tested compound are added
Initial conc.:
200 mg/L
Based on:
COD
Parameter followed for biodegradation estimation:
other: COD (Organic Carbon)
Details on study design:
- Composition of medium: In cq. 800 ml of distilled water solutions of calcium chloride (27.5 g CaCI2 in 1 L distilled water), magnesium sulphate (22.5 g MgSO4. 7 H20 in 1L distilled water), and ferric chloride (0.25 g FeCI3; 6 H20 in 1L distilled water) are added in 1-ml portions each. Then 5 ml ammonium sulphate solution [10g (NH4)2.SO4in 1 L distilled water], 20ml of a phosphate buffer of pH 7.2 (8.5 g KH2PO4, 21.8g K2HPO4, and 44.7g Na2HPO4.12 H2O in 1 L distilled water), and 100ml of tap water for securing the content of trace elements, are added. The solution thus prepared is made up to 1000ml with distilled water. - Test temperature: 20 ± 3 oC- Continuous darkness: yes- Sampling method: Samples filtered or centrifuged before analysisCONTROL AND BLANK SYSTEM- Inoculum blank
Key result
Parameter:
other: % degradation by COD
Value:
59.4
Remarks on result:
other: other details not available
Details on results:
Percent biodegradability of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate observed to be 59.4 % by COD (Organic Carbon) parameter and he rate of biodegradation observed was 0.8 mg COD/g/h using adapted activated sludge as inoculums at 20 oC.
Validity criteria fulfilled:
not specified
Interpretation of results:
inherently biodegradable
Conclusions:
Percent biodegradability of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate observed to be 59.4 % by COD ( Organic Carbon) parameter using adapted activated sludge as inoculums at 20 oC . Thus it is concluded that test chemical Bis(4-hydroxy-N-methylanilinium) sulphate is inherently biodegradable.
Executive summary:

Biodegradation experiment was performed in batch system using adapted activated sludge as inoculums. Activated sludge was obtained from sewage plant then it is adapted for 20 days with the test chemical Bis(4-hydroxy-N-methylanilinium) sulphate ( CAS no. 55-55-0) in biological medium containing Glucose peptone etc. After activated sludge get adapted concentration of test chemical was increased upto 200 mg/L and then biodegrdation was observed for test chemical. Test chemical was the only source of organic carbon. All adaption process and degradation process was taken place in dark room at 20 oC. Experiment was carried out till there is no decrease of COD. Inoculum blank flask was also set up simultaneously. Sampling was done at definite time interval 50-80 mL sample was taken for analyzing COD. The experiment is carried out till there is no decrease of COD. After that time the total percentage of COD removed (% degradation) and the rate of degradation are evaluated. Percent biodegradability of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate observed to be 59.4 % by COD (Organic Carbon ) and rate of degradation was 0.8 mg COD /g/L per hour. If the degradation rate being over 15 mg of COD g /L/h), the substance tested can be considered to be biologically readily decomposable but for test chemical it was only 0.8mg COD g/L/h. Thus it is concluded that test chemical Bis(4-hydroxy-N-methylanilinium) sulphate is inherently biodegradable.

Description of key information

Biodegradation experiment was performed in batch system using adapted activated sludge as inoculums. Activated sludge was obtained from sewage plant then it is adapted for 20 days with the test chemical Bis(4-hydroxy-N-methylanilinium) sulphate (CAS no. 55-55-0) in biological medium containing Glucose peptone etc. After activated sludge get adapted concentration of test chemical was increased upto 200 mg/L and then biodegrdation was observed for test chemical. Test chemical was the only source of organic carbon. All adaption process and degradation process was taken place in dark room at 20 oC. Experiment was carried out till there is no decrease of COD. Inoculum blank flask was also set up simultaneously. Sampling was done at definite time interval 50-80 mL sample was taken for analyzing COD. The experiment is carried out till there is no decrease of COD. After that time the total percentage of COD removed (% degradation) and the rate of degradation are evaluated. Percent biodegradability of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate observed to be 59.4 % by COD (Organic Carbon) and rate of degradation was 0.8 mg COD /g/L per hour. If the degradation rate being over 15 mg of COD g /L/h), the substance tested can be considered to be biologically readily decomposable but for test chemical it was only 0.8 mg COD g/L/h. Thus it is concluded that test chemical Bis(4-hydroxy-N-methylanilinium) sulphate is inherently biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable

Additional information

Biodegradation experiment was performed in batch system using adapted activated sludge as inoculums. Activated sludge was obtained from sewage plant then it is adapted for 20 days with the test chemical Bis(4-hydroxy-N-methylanilinium) sulphate (CAS no. 55-55-0) in biological medium containing Glucose peptone etc. After activated sludge get adapted concentration of test chemical was increased upto 200 mg/L and then biodegrdation was observed for test chemical. Test chemical was the only source of organic carbon. All adaption process and degradation process was taken place in dark room at 20 oC. Experiment was carried out till there is no decrease of COD. Inoculum blank flask was also set up simultaneously. Sampling was done at definite time interval 50-80 mL sample was taken for analyzing COD. The experiment is carried out till there is no decrease of COD. After that time the total percentage of COD removed (% degradation) and the rate of degradation are evaluated. Percent biodegradability of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate observed to be 59.4 % by COD (Organic Carbon) and rate of degradation was 0.8 mg COD /g/L per hour. If the degradation rate being over 15 mg of COD g /L/h), the substance tested can be considered to be biologically readily decomposable but for test chemical it was only 0.8mg COD g/L/h. Thus it is concluded that test chemical Bis(4-hydroxy-N-methylanilinium) sulphate is inherently biodegradable.