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Environmental fate & pathways

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Biodegradation in water:

Biodegradation experiment was performed in batch system using adapted activated sludge as inoculums. Activated sludge was obtained from sewage plant then it is adapted for 20 days with the test chemical Bis(4-hydroxy-N-methylanilinium) sulphate ( CAS no. 55-55-0) in biological medium containing Glucose peptone etc. After activated sludge get adapted concentration of test chemical was increased upto 200 mg/L and then biodegrdation was observed for test chemical. Test chemical was the only source of organic carbon. All adaption process and degradation process was taken place in dark room at 20 oC. Experiment was carried out till there is no decrease of COD. Inoculum blank flask was also set up simultaneously. Sampling was done at definite time interval 50-80 mL sample was taken for analyzing COD. The experiment is carried out till there is no decrease of COD. After that time the total percentage of COD removed (% degradation) and the rate of degradation are evaluated. Percent biodegradability of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate observed to be 59.4 % by COD (Organic Carbon ) and rate of degradation was 0.8 mg COD /g/L per hour. If the degradation rate being over 15 mg of COD g /L/h), the substance tested can be considered to be biologically readily decomposable but for test chemical it was only 0.8mg COD g/L/h. Thus it is concluded that test chemical Bis(4-hydroxy-N-methylanilinium) sulphate is inherently biodegradable.

Biodegradation in water and sediments:

Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound Bis(4-hydroxy-N-methylanilinium) sulphate (CAS No. 55-55-0). If released in to the environment, 12 % of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 37.5 days (900 hrs). The half-life (37.5 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is low whereas the half-life period of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate in sediment is estimated to be 337.5 days (8100 hrs). However, as the percentage release of test chemical is less than 1 % (i.e.,reported as 0.55) indicates that the test chemical is not persistent in sediments.

Biodegradation in Soil:

The half-life period of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate (CAS No. 55 -55 -0) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 87.5 % of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical Bis(4-hydroxy-N-methylanilinium) sulphat ein soil is estimated to be 75 days (1800 hrs). Based on this half-life value of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is low.

Additional information

Biodegradation in water:

Biodegradation experiment was performed in batch system using adapted activated sludge as inoculums. Activated sludge was obtained from sewage plant then it is adapted for 20 days with the test chemical Bis(4-hydroxy-N-methylanilinium) sulphate ( CAS no. 55-55-0) in biological medium containing Glucose peptone etc. After activated sludge get adapted concentration of test chemical was increased upto 200 mg/L and then biodegrdation was observed for test chemical. Test chemical was the only source of organic carbon. All adaption process and degradation process was taken place in dark room at 20 oC. Experiment was carried out till there is no decrease of COD. Inoculum blank flask was also set up simultaneously. Sampling was done at definite time interval 50-80 mL sample was taken for analyzing COD. The experiment is carried out till there is no decrease of COD. After that time the total percentage of COD removed (% degradation) and the rate of degradation are evaluated. Percent biodegradability of test chemical Bis(4-hydroxy-N-methylanilinium) sulphate observed to be 59.4 % by COD (Organic Carbon ) and rate of degradation was 0.8 mg COD /g/L per hour. If the degradation rate being over 15 mg of COD g /L/h), the substance tested can be considered to be biologically readily decomposable but for test chemical it was only 0.8mg COD g/L/h. Thus it is concluded that test chemical Bis(4-hydroxy-N-methylanilinium) sulphate is inherently biodegradable.